HTS233LRTA
Ready-to-Assay LH Glycoprotein Hormone Receptor Frozen Cells
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About This Item
UNSPSC Code:
41106514
eCl@ss:
32011203
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General description
EMD Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. EMD Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
EMD Millipore’s ChemiBrite cells co-express a GPCR along with a novel variant of clytin, a calcium-activated photoprotein, to enable sensitive luminescent detection of ligand-induced calcium flux. The ChemiBrite version of clytin contains a mutation that increases its affinity for calcium to a level that permits detection of cytosolic calcium in many cells with greater sensitivity than other mitochondrially expressed photoproteins. Luminescent calcium assays offer several advantages over fluorescent calcium assays including; lower substrate cost, increased sensitivity, and lack of interference from fluorescent compounds.
Luteinizing Hormone (LH), also known as lutropin, is produced by the pituitary gland and is critical for fertility and reproduction. In women, LH modulates ovulation. In men, LH stimulates testosterone production. Several naturally occurring mutations in the LH receptor gene have been associated with human reproductive disorders (Themmen et al.). Together with TSH, hCG and FSH, LH is a member of the glycoprotein hormone family. Each member shares a common α subunit, whereas the β subunit confers functional specificity. Upon activation, LH signals primarily through the Gαqs/adenylyl cyclase/cAMP/PKA pathway as has been shown in native granulosa cells. EMD Millipore’s cloned human LH receptor-expressing ChemiBrite cells were made by recombinant transfection of HEK293 cells with ChemiBrite clytin, the LH receptor and a promiscuous G protein to couple the receptor to the calcium signaling pathway. The cells have been cryopreserved at an optimal time post-transfection. Upon thaw, recovery, and loading, the cells are ready for luminescent, fluorescent and cAMP accumulation analysis of agonists, antagonists and modulators at the LH receptor.
EMD Millipore’s ChemiBrite cells co-express a GPCR along with a novel variant of clytin, a calcium-activated photoprotein, to enable sensitive luminescent detection of ligand-induced calcium flux. The ChemiBrite version of clytin contains a mutation that increases its affinity for calcium to a level that permits detection of cytosolic calcium in many cells with greater sensitivity than other mitochondrially expressed photoproteins. Luminescent calcium assays offer several advantages over fluorescent calcium assays including; lower substrate cost, increased sensitivity, and lack of interference from fluorescent compounds.
Luteinizing Hormone (LH), also known as lutropin, is produced by the pituitary gland and is critical for fertility and reproduction. In women, LH modulates ovulation. In men, LH stimulates testosterone production. Several naturally occurring mutations in the LH receptor gene have been associated with human reproductive disorders (Themmen et al.). Together with TSH, hCG and FSH, LH is a member of the glycoprotein hormone family. Each member shares a common α subunit, whereas the β subunit confers functional specificity. Upon activation, LH signals primarily through the Gαqs/adenylyl cyclase/cAMP/PKA pathway as has been shown in native granulosa cells. EMD Millipore’s cloned human LH receptor-expressing ChemiBrite cells were made by recombinant transfection of HEK293 cells with ChemiBrite clytin, the LH receptor and a promiscuous G protein to couple the receptor to the calcium signaling pathway. The cells have been cryopreserved at an optimal time post-transfection. Upon thaw, recovery, and loading, the cells are ready for luminescent, fluorescent and cAMP accumulation analysis of agonists, antagonists and modulators at the LH receptor.
Cell Line Description
GPCR Cell Lines
HEK293
Biochem/physiol Actions
GPCR Class: A
Protein Target: LH
Target Sub-Family: Glycoprotein hormone
Components
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
Fifty (50) mL of Media Component.
Fifty (50) mL of Media Component.
Legal Information
GenBank is a registered trademark of United States Department of Health and Human Services
Disclaimer
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges {HCompany} to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Themmen APN et al.
Endocrine reviews, 21(5), 551-583 (2000-10-21)
The recent unraveling of structures of genes for the gonadotropin subunits and gonadotropin receptors has provided reproductive endocrinologists with new tools to study normal and pathological functions of the hypothalamic-pituitary-gonadal axis. Rare inactivating mutations that produce distinctive phenotypes of isolated
X C Jia et al.
Molecular endocrinology (Baltimore, Md.), 5(6), 759-768 (1991-06-01)
Studies on human LH receptors are difficult due to the limited availability of clinical samples. Recent cloning of rat and porcine LH receptor cDNAs indicated that these binding sites are single polypeptides of the G-protein-coupled receptor family with seven transmembrane
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