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Key Documents

ABT63

Sigma-Aldrich

Anti-Ena/VASP-like protein Antibody

from rabbit, purified by affinity chromatography

Sinónimos:

Ena/VASP-like protein, Ena/vasodilator-stimulated phosphoprotein-like

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse

species reactivity (predicted by homology)

rat (based on 100% sequence homology), chimpanzee (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... EVL(51466)

General description

Ena (VASP-like protein) is involved in many different cellular functions including cell migration and axon guidance. Studies have shown that Ena expression is significantly increased in breast cancer and that the level of expression is associated with tumor stage. Ena has also been linked to inner ear and hair cell development.

Specificity

This antibody recognizes Ena/VASP-like protein at the EVH2 region.

Immunogen

Epitope: EVH2 region
KLH-conjugated linear peptide corresponding to the EVH2 region of human Ena/VASP-like protein.

Application

Detect Ena/VASP-like protein using this Anti-Ena/VASP-like protein Antibody validated for use in WB & IC.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Ena/VASP-like protein in NIH/3T3 cells.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton

Quality

Evaluated by Western Blot in Ramos cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Ena/VASP-like protein in 10 µg of Ramos cell lysate.

Target description

~46 kDa observed.

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Ramos cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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