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Key Documents

ABS2208

Sigma-Aldrich

Anti-GPR161

Sinónimos:

G-protein coupled receptor 161

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

mol wt

calculated mol wt 60.3 kDa
observed mol wt ~60. kDa

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

mouse

packaging

antibody small pack of 100

technique(s)

immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

epitope sequence

C-terminal

Protein ID accession no.

UniProt accession no.

storage temp.

-10 to -25°C

Gene Information

mouse ... Gpr161(240888)

Specificity

This rabbit polyclonal antibody detects G-protein coupled receptor 161 (GPR161). It targets an epitope within 28 amino acids from the C-terminal, cytoplasmic domain.

Immunogen

KLH-conjugated linear peptide corresponding to 28 amino acids from the C-terminal, cytoplasmic domain of mouse G-protein coupled receptor 161 (GPR161).

Application

Quality Control Testing

Evaluated by Western Blotting in lysate from HEK293T cell transiently overexpressing human GPR161

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GPR161 in lysate from HEK293T cell transiently overexpressing human GPR161.

Tested Applications

Immunofluorescence Analysis: A representative lot detected GPR161 in Immunofluorescence application (Shimada, H., et. al. (2017). Cell Rep. 20(2):384-396).

Immunoprecipitation Analysis: A representative lot immunoprecipitated GPR161 in Immunoprecipitation application (Mukhopadhyay, S., et. al. (2013). Cell. 152(1-2):210-23).

Immunocytochemistry Analysis: A representative lot detected GPR161 in Immunocytochemistry application (Mukhopadhyay, S., et. al. (2013). Cell. 152(1-2):210-23).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

G-protein coupled receptor 161 (UniProt: B2RPY5; also known as GPR161) is encoded by the Gpr161 (also known as Gm208) gene (Gene ID: 240888) in murine species. GPR161 is a multi-pass membrane glycoprotein of the GPCR family with seven transmembrane domains. It localizes to the ciliary membrane and is also detected in the recycling endocytic compartment. It is ubiquitously expressed from 8.5 dpc and is mostly concentrated in the developing nervous system at later stages. By 10.5 dpc, it is mainly expressed in the neural tube. At later embryonic stages it is predominantly expressed in the brain, spinal cord, and dorsal ganglia. GPR161 functions as a negative regulator of sonic hedgehog (Shh) signaling, which promotes the processing of Gli3 into its repressive form (Gli3R) during neural tube development via cAMP-protein kinase A signaling. In the presence of Shh, it is removed from primary cilia and is internalized into recycling endosomes, preventing its activity, and allowing activation of the Shh signaling. Gpr161 knockout mice display increased Shh signaling and exhibit embryonic lethality by 10.5 dpc. GPR161 is overexpressed in triple-negative breast cancer and serves is a regulator of mammary epithelial cell proliferation and invasion in a mTOR-dependent manner. Knockdown of GPR161 is reported to impair proliferation of human basal breast cancer cell lines and its overexpression increases cell proliferation, migration, and intracellular accumulation of E-cadherin. Three isoforms of GPR161 have been described that are produced by alternative splicing. (Ref.: Hwang, S-H., et al. (2021). eLife 10:e67121; Mukhopadhyay, S., et al. (2013). Cell 152(1-2); 210-223; Feigin, ME., et al. (2014). Proc. Natl. Acad. Sci. USA. 111(11); 4191-4196).

Physical form

Purified rabbit polyclonal antibody in PBS without azide.

Reconstitution

0.5 mg/mL Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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