ABE1402
Anti-LHX2 Antibody
serum, from rabbit
Sinónimos:
LIM/homeobox protein Lhx2, Homeobox protein LH-2, LIM homeobox protein, LHX2
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43
Productos recomendados
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human
technique(s)
ChIP: suitable (ChIP-seq)
immunofluorescence: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
mouse ... Lhx2(16870)
General description
LIM/homeobox protein Lhx2 (UniProt Q9Z0S2; also known as Homeobox protein LH-2, LIM homeo box protein 2, Lim2) is encoded by the Lhx2 gene (Gene ID 16870) in murine species. Lhx2 is a transcription factor that plays an important role in controlling the behavior of progenitor cells involved in the development of the skin hair cycling, eye, forebrain, limb, hematopoietic, olfactory, and pituitary systems. Lhx2 is shown to regulate a significant number of hair follicle stem cell (HF-SC) signature genes, as well as cytoskeletal and adhesion molecules within the niche where HF-SCs reside as a single layer of polarized, quiescent epithelial cells. Lhx2 deficiency leads to baldness, because the niche can no longer anchor its hair and maintain the proper behavior of its SCs as a result of greatly perturbed HF-SCs polarization and niche architecture.
Immunogen
Recombinant protein corresponding to mouse LHX2.
Application
Immunofluorescence Analysis: A representative lot detected Lhx2 immunoreactivity by fluorescent immunohistochemistry using OCT-embedded, PFA-fixed backskin cryosections from wild-type, but not Lhx2 knockout, mice at varies developmental stages, including E16.5, P0, P30, and the second telogen (Folgueras, A.R., et. al. (2013). Cell Stem Cell. 13(3):314-327).
Chromatin Immunoprecipitation Analysis: A representative lot detected Lhx2-targeted promoter and enhancer regions using α6hi/CD34+ skin cells enriched in hair follicle stem cells (HF-SCs) from mice at the second telogen developmental stage (Folgueras, A.R., et. al. (2013). Cell Stem Cell. 13(3):314-327).
Chromatin Immunoprecipitation-Sequencing Analysis: A representative lot detected Lhx2-targeted promoter and enhancer regions by a genome-wide ChIP-seq analysis using α6hi/CD34+ skin cells enriched in hair follicle stem cells (HF-SCs) from mice at the second telogen developmental stage (Folgueras, A.R., et. al. (2013). Cell Stem Cell. 13(3):314-327).
Immunofluorescence Analysis: A representative lot (1:1,000 dilution) detected Lhx2 immunoreactivity in human skin samples by fluorescent immunohistochemistry using OCT-embedded, PFA-fixed cryosections (Data courtesy of Dr. Elaine Fuchs, Howard Hughes Medical Institute, The Rockefeller University ).
Chromatin Immunoprecipitation Analysis: A representative lot detected Lhx2-targeted promoter and enhancer regions using α6hi/CD34+ skin cells enriched in hair follicle stem cells (HF-SCs) from mice at the second telogen developmental stage (Folgueras, A.R., et. al. (2013). Cell Stem Cell. 13(3):314-327).
Chromatin Immunoprecipitation-Sequencing Analysis: A representative lot detected Lhx2-targeted promoter and enhancer regions by a genome-wide ChIP-seq analysis using α6hi/CD34+ skin cells enriched in hair follicle stem cells (HF-SCs) from mice at the second telogen developmental stage (Folgueras, A.R., et. al. (2013). Cell Stem Cell. 13(3):314-327).
Immunofluorescence Analysis: A representative lot (1:1,000 dilution) detected Lhx2 immunoreactivity in human skin samples by fluorescent immunohistochemistry using OCT-embedded, PFA-fixed cryosections (Data courtesy of Dr. Elaine Fuchs, Howard Hughes Medical Institute, The Rockefeller University ).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Nuclear Receptors
This Anti-LHX2 Antibody is validated for use in Western Blotting, Immunofluorescence, ChIP-seq and Chromatin Immunoprecipitation (ChIP) for the detection of LHX2.
Quality
Evaluated by Western Blotting in Murine keratinocyte K14rtTA-LHX2-TRE cell lysate.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected doxycycline-induced LHX2 expression in 10 µg of Murine keratinocyte K14rtTA-LHX2-TRE cell lysate.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected doxycycline-induced LHX2 expression in 10 µg of Murine keratinocyte K14rtTA-LHX2-TRE cell lysate.
Target description
~48 kDa detected. Target band size appears larger than the calculated molecular weight of 44.4 kDa due to posttranslational glycosylation. Uncharacterized band(s) may appear in some lysates.
Physical form
Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Alicia R Folgueras et al.
Cell stem cell, 13(3), 314-327 (2013-09-10)
In adult skin, self-renewing, undifferentiated hair follicle stem cells (HF-SCs) reside within a specialized niche, where they spend prolonged times as a single layer of polarized, quiescent epithelial cells. When sufficient activating signals accumulate, HF-SCs become mobilized to fuel tissue
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