ABC1444

Anti-Sorting Nexin-11 Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: Sorting nexin-11, SNX11
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, human
• species reactivity (predicted by homology): rat (based on 100% sequence homology)
• technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• NCBI accession no.: NP_001156861
• UniProt accession no.: Q91WL6
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Snx11(74479)

Descripción

General description

Sorting nexin-11 (UniProt Q91WL6) is encoded by the Snx11 gene (Gene ID 74479) in murine species. Sorting nexins (SNXs) are involved in diverse intracellular endosomal traffickings. Their phox homology (PX) domain binds certain phosphatidylinositols (PIs) and mediates their recruitment to PI-rich vesicles. Most SNXs contain additional recognizable domains and are referred to as SNX PX-BAR or SNX PX-other, while SNX3, SNX10, SNX11, SNX12, SNX22, and SNX24 contain only the PX domain and are referred to as SNX PX. SNX11 is found to contain a novel PXe domain (a.a. 16-132 of human and mouse SNX11) with two additional α-helices next to the conventional PX domain structure composed of three β-strands and three α-helices. F2RL1 (a.k.a. PAR2) is a protease-activated GPCR highly expressed throughout the central and the peripheral nervous system. F2RL1 is reported to translocate from the plasma membrane to nucleus upon stimulation via a microtubule-dependent shuttle machinery that requires sorting nexin 11.

Specificity

This polyclonal antibody reacts with both human and murine sorting nexin-11. The immunogen sequence is also present in human spliced isoform 2 and rat sorting nexin-11.

Immunogen

Epitope: Internal (C-terminal half).

Linear peptide corresponding to an internal sequence from the C-terminal half of mouse sorting nexin-11.

Application

Anti-Sorting Nexin-11 Antibody is an antibody against Sorting Nexin-11 for use in Western Blotting, Immunoprecipitation.

Research Category
Apoptosis & Cancer

Research Sub Category
Vesicular Trafficking

Western Blotting Analysis: 3.75 µg/mL from a representative lot detected sorting nexin-11 in 10 µg of HEK293T cell lysate.
Western Blotting Analysis: A representative lot detected reducing amount of sorting nexin-11 in lysates from HEK293 cells that had been transfected with SNX11 shRNA (Joyal, J.S., et al. (2014). Nat. Med. 20(10):1165-1173).
Western Blotting Analysis: A representative lot detected the presence of sorting nexin-11 in F2RL1-GFP fusion affinity pull-down only when using lysates of F2RL1-GFP-transfected HEK293 cells that had been stimulated with the F2RL1 agonist peptide (F2RL1-AP) SLIGKV, but not unstimulated F2RL1-GFP transfectants (Joyal, J.S., et al. (2014). Nat. Med. 20(10):1165-1173).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated importin-β1 (Impβ1) and F2RL1 with sorting nexin-11 from lysates of HEK293 cells that had been stimulated with the F2RL1 agonist peptide (F2RL1-AP) SLIGKV (Joyal, J.S., et al. (2014). Nat. Med. 20(10):1165-1173).

Quality

Evaluated by Western Blotting in MCF-7 membrane extract.

Western Blotting Analysis: A 1:500 dilution of this antibody detected sorting nexin-11 in 10 µg of MCF-7 membrane extract.

Target description

~35 kDa observed. 30.43 kDa (human & mouse isoform 1) and 29.42 kDa (human isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

Physical form

Affinity purified

Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable