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Key Documents

AB15708C3

Sigma-Aldrich

Anti-Beta III Tubulin, Cy3 Conjugate Antibody

from rabbit, CY3 conjugate

Sinónimos:

Anti-CDCBM, Anti-CDCBM1, Anti-CFEOM3, Anti-CFEOM3A, Anti-FEOM3, Anti-TUBB4, Anti-beta-4

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

CY3 conjugate

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat

species reactivity (predicted by homology)

mouse (based on 100% sequence homology), human (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TUBB3(10381)
mouse ... Tubb3(22152)

General description

Tubulin is one of several members of a small family of globular proteins which polymerize to form cytoskeletal structures called microtubules. The most common members of the tubulin family are α-tubulin and β-tubulin. Tubulin dimers, typically composed of α- and β-tubulin subunits, assemble onto microtubules forming large polymers. β-III tubulin is a unique microtubule subunit that is found expressed almost exclusively in neurons. Due to the unique expression pattern of β-III tubulin it is often utilized as a marker to identify neuronal cells from other brain cells such as glia which do not express it. β-III tubulin also serves as a cytoarchitectural marker since it is primarily found within neurite extensions.

Immunogen

Synthetic linear peptide corresponding to human Beta III Tubulin.

Application

Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Neurofilament & Neuron Metabolism
This Anti-Beta III Tubulin, Cy3 Conjugate Antibody is validated for use in ICC for the detection of Beta III Tubulin.

Quality

Evaluated by Immunocytochemistry in rat E18 cortex cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Beta III Tubulin in rat E18 cortex cells.

Target description

50 kDa calculated

Physical form

Affinity purified
Purified rabbit polyclonal conjugated to Cy3 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rat E18 cortex cells

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

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Visite la Librería de documentos

Andrew J Matamoros et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 39(11), 2011-2024 (2019-01-17)
Fidgetin is a microtubule-severing protein that pares back the labile domains of microtubules in the axon. Experimental depletion of fidgetin results in elongation of the labile domains of microtubules and faster axonal growth. To test whether fidgetin knockdown assists axonal

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