475954

MRN-ATM Pathway Inhibitor, Mirin

The MRN-ATM Pathway Inhibitor, Mirin, also referenced under CAS 299953-00-7, controls the biological activity of Mre11, component of the MRN (Mre11-Rad50-Nbs1) complex. This small molecule/inhibitor is primarily used for Cell Structure applications.

• Sinónimos: MRN-ATM Pathway Inhibitor, Mirin, (Z)-5-(4-Hydroxybenzylidene)-2-imino-1,3-thiazolidin-4-one, Mirin
• Fórmula empírica (notación de Hill): C₁₀H₈N₂O₂S
• Número de CAS: 299953-00-7
• Peso molecular: 220.25
• MDL number: MFCD05885480
• UNSPSC Code: 12352200
• NACRES: NA.77

Propiedades

• Quality Level: 100
• assay: ≥95% (HPLC)
• form: powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: red-orange
• solubility: DMSO: 10 mg/mL; ethanol: 3 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C

Descripción

General description

A cell-permeable pyrimidinone compound that inhibits the exonuclease activity of Mre11, component of the MRN (Mre11-Rad50-Nbs1) complex, and prevents MRN-mediated activation of dimeric ATM (IC₅₀ = 12 µM) in cell-free p53 Ser15 phosphorylation assays, while exhibiting little effect against MRN-independent ATM activation in the presence of high DSBs (DNA double strain breaks) or the activity of activated monomeric ATM. Mirin is shown to cause an increase of G2 population in exponentially growing TOSA4 cultures (~1.6-fold at 50 µM) and dramatically increase mitotic cell polulation (~10-fold at 25 µM) after irradiation of G2 phase U2OS cultures by preventing IR-induced G2/M checkpoint. Mirin exhibits no inhibitory activity against Rad50-associated adenylate kinase or Exonulease III.

A cell-permeable pyrimidinone compound that inhibits the exonuclease activity of Mre11, component of the MRN (Mre11-Rad50-Nbs1) complex, and prevents MRN-mediated activation of dimeric ATM (IC₅₀ = 12 µM) in cell-free p53 Ser15 phosphorylation assays, while exhibiting little effect against MRN-independent ATM activation in the presence of high DSBs (DNA double strain breaks) or the activity of activated monomeric ATM. Mirin is shown to cause an increase of G2 population in exponentially growing TOSA4 cultures (~1.6-fold at 50 µM) and dramatically increase mitotic cell polulation (~10-fold at 25 µM) after irradiation of G2 phase U2OS cultures by preventing IR-induced G2/M checkpoint. Mirin exhibits no inhibitory activity against Rad50-associated adenylate kinase or Exonulease III.

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20&degC.

Other Notes

Garner, K.M., et al. 2009. Nature Chem. Biol.5, 129.
Peterson, S., et al. 2009. Nature Chem. Biol.5, 130.
Dupre, A., et al. 2008. Nature Chem. Biol.4, 119.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable