422426

KGF/FGF-7, Mouse, Recombinant

• UNSPSC Code: 12352202
• NACRES: NA.77

Propiedades

• assay: ≥90% (SDS-PAGE)
• Quality Level: 100
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• impurities: <1.0 EU/μg protein Endotoxin
• shipped in: ambient
• storage temp.: −20°C

Descripción

General description

Recombinant, mouse keratinocyte growth factor (KGF) consisting of amino acids 32-194 and expressed in E. coli. KGF plays an essential role in development, tumorigenesis, angiogenesis, morphogenesis, and wound healing. Mature mouse KGF amino acid sequence is 96% identical with rat, bovine, ovine, equine, and human forms and is active across species.

Biochem/physiol Actions

ED₅₀ = 10-50 ng/ml as measured in a cell proliferation assay using 4MBr-5 monkey epithelial cells.

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 0.2 µm sterile-filtered MOPS, Na₂SO₄, EDTA, DTT BSA.

Reconstitution

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 250 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.

Other Notes

Belleudi, F., et al. 2007. Traffic8, 1854.
Finch, P.W. and J.S. Rubin. 2006. J. Natl. Cancer Inst.98, 812.
de Georgi, V., et al. 2007. Dermatol. Clin.25, 477.
Niu, J., et al. 2007. J. Biol. Chem.282, 6001.
Werner, S., et al. 2007. J. Invest. Dermatol.127, 998.
Cardinali, G., et al. 2005. J. Invest. Dermatol.125, 1190.
Geer, D.J., et al. 2005. Am. J. Pathol.167, 1575.
Hsu, YR., et al. 1999. Biochemistry38, 2523.
Qiao, J., et al. 1999. Development126, 547.
Werner, S., 1998. Cytokine Growth Factor Rev.9, 153.
Guo, L., et al. 1996. Genes Dev.10, 165.
Mason, I.J., et al. 1994. Mech. Dev.45, 15.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2