218765

Caspase-9 Substrate I, Fluorogenic

A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6).

• Sinónimos: Caspase-9 Substrate I, Fluorogenic, Ac-LEHD-AFC
• Fórmula empírica (notación de Hill): C₃₃H₃₈F₃N₇O₁₁
• Número de CAS: 210345-03-2
• Peso molecular: 765.69
• MDL number: MFCD01862610
• UNSPSC Code: 12352200
• NACRES: NA.77

Propiedades

• Quality Level: 100
• assay: ≥95% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated; protect from light
• color: white
• solubility: DMSO: 5 mg/mL
• fluorescence: λ_ex ~400 nm; λ_em ~505 nm
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: FC(F)(F)C1=CC(=O)Oc2c1ccc(c2)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)C)CC(C)C)CCC(=O)O)Cc3[nH]cnc3)CC(=O)O
• InChI: 1S/C33H38F3N7O11/c1-15(2)8-22(39-16(3)44)31(52)41-21(6-7-26(45)46)29(50)42-23(9-18-13-37-14-38-18)32(53)43-24(12-27(47)48)30(51)40-17-4-5-19-20(33(34,35)36)11-28(49)54-25(19)10-17/h4-5,10-11,13-15,21-24H,6-9,12H2,1-3H3,(H,37,38)(H,39,44)(H,40,51)(H,41,52)
• InChI key: HULKIXRFKRCRHD-ZJZGAYNASA-N

Descripción

General description

A fluorogenic substrate for caspase-9 (ICE-LAP6, Mch6). May also act as a substrate for caspase-4 and caspase-5. Reaction can be monitored visually or quantitatively by a blue to green shift in fluorescence upon cleavage of the AFC fluorophore.

Biochem/physiol Actions

Cell permeable: no

Primary Target
A fluorogenic substrate for caspase-9

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Standard Handling (A)

Sequence

Ac-Leu-Glu-His-Asp-AFC

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Ito, A., et al. 1999. Biochim. Biophys. Acta 1452, 263.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable