17-327

H2A.X Phosphorylation Assay Kit (Chemiluminescence Detection)

• UNSPSC Code: 12161503
• NACRES: NA.54

Propiedades

• packaging: kit of 192 assay(s)
• Quality Level: 100
• manufacturer/tradename: Upstate^®
• technique(s): ELISA: suitable
• detection method: chemiluminescent

Descripción

General description

Phosphorylation of the histone variant H2A.X is a rapid and sensitive response to double strand DNA breaks. The H2A.X Phosphorylation Assay Kit (Chemiluminescence Detection) is a cell based assay formatted for microplate-based detection of levels of phosphorylated histone H2A.X.

Application

The H2A.X Phosphorylation, Chemiluminescence Detection Assay Kit is a cell-based ELISA formatted for chemiluminescent detection of relative levels of phosphorylated H2A.X in microplate cells cultures.

Components

• Anti-phospho-Histone H2A.X (Ser139), clone JBW301 (Cat.# 05-636)
• TBS, 20X (Cat.# 20-190)
• 20% Tween-20 (v/v)
• 10% BSA in TBS (Blocking Buffer)
• LumiGLO Substrate Reagent A
• LumiGLO Substrate Reagent B
• Goat Anti-Mouse IgG, HRP conjugate

Quality

The H2A.X Phosphorylation, Chemiluminescence Detection Assay Kit is a cell-based ELISA formatted for chemiluminescent detection of relative levels of phosphorylated H2A.X in microplate cells cultures. Adherent cells are cultured in microplates, treated with agents that induce DNA damage/H2A.X phosphorylation, and are then fixed and permeabilized. Histone H2A.X phosphorylated at serine 139 is detected by Anti-phospho-H2A.X (Ser139), clone JBW301 and an anti-mouse-HRP (horseradish peroxidase) conjugate. The chemiluminescent HRP substrate LumiGLO is then added, and signal is measured in a microplate luminometer

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.