14-843-M

Hck Protein, activated

• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

Propiedades

• Quality Level: 100; 300
• recombinant: expressed in baculovirus infected Sf21 cells
• description: protein target Hck
• purified by: (Ni²⁺/NTA-agarose)
• technique(s): activity assay: suitable
• UniProt accession no.: P08631
• Gene Information: human ... HCK(3055)

Descripción

General description

FUNCTION: SwissProt: P08631 # May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.
SIZE: 526 amino acids; 59600 Da
SUBUNIT: May bind to HIV-1 Nef and VIF through its SH3 domain. This interaction would stimulates its tyrosine-kinase activity.
SUBCELLULAR LOCATION: Isoform p59-HCK: Membrane; Lipid-anchor. & Isoform p60-HCK: Membrane; Lipid-anchor. Cytoplasm.
TISSUE SPECIFICITY: Expressed predominantly in cells of the myeloid and B-lymphoid lineages.
DOMAIN: SwissProt: P08631 The SH3 domain mediates binding to HIV-1 Nef.
PTM: Isoform p59-HCK contains a N-myristoyl glycine at position 3 (By similarity). Isoform p59-HCK contains a S-palmitoyl cysteine at position 3 (By similarity).
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.

Application

Auto-activated on column by incubating with Mg/ATP, excess ATP and MgAc removed by multiple column wash steps.

Packaging

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-843M when ordering the 250 µg size.

Also available in 10 μg size (cat#14-843) and in bulk (cat# 14-843-K).

Physical properties

34.1kDa

Physical form

50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol, 5mM β-glycerophosphate, 1mM Na3VO4. Frozen solution.

Storage and Stability

6 months at -70°C

Analysis Note

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable