05-824

Anti-Estrogen Receptor β Antibody, clone 68-4, rabbit monoclonal

clone 68-4, Upstate^®, from rabbit

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• biological source: rabbit
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 68-4, monoclonal
• species reactivity: human
• packaging: antibody small pack of 25 μL
• manufacturer/tradename: Upstate^®
• technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_001437
• UniProt accession no.: Q92731
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... ESR2(2100)

Descripción

General description

Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ERα and ERβ, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ERα and ERβ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERα and ERβ may be regulated by distinct mechanisms even though they share many functional characteristics.

Produced in collaboration with Epitomics

Specificity

Predicted to cross-react with rat and mouse based on sequence homology

Recognizes Estrogen Receptor β, long form, does not detect estrogen receptor alpha.

Immunogen

KLH-conjugated linear peptide corresponding to amino acids 108-127 from the N-terminal half of human estrogen receptor beta.

Application

Detect Estrogen Receptor β also known as Nuclear receptor subfamily 3 group A member 2 with Anti-Estrogen Receptor β Antibody, clone 68-4 (Rabbit Monoclonal Antibody), that has been demonstrated to work in IP & WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Quality

routinely evaluated by immunoblot on RIPA lysates from MCF7 cells

Target description

59 kDa

Physical form

Format: Purified

Protein A Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl and 0.05% sodium azide, pH 7.4.

Protein A purified

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
MCF7 cell extracts

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1