Todas las fotos(2)

Documentos clave

05-785

Anti-CD3ε Antibody, clone APA1/1

Name: Anti-CD3ε Antibody, clone APA1/1
Brand: MM

clone APA1/1, Upstate^®, from mouse

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización

Todas las fotos(2)

About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

APA1/1, monoclonal

species reactivity

mouse, human

manufacturer/tradename

Upstate^®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_000733

UniProt accession no.

P07766

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CD3E(916)

Specificity

CDε

Immunogen

Purified human CD3 epsilon

Application

Clone APA1/1 has been demonstrated to recognize a site on CD3&epsilon: that is exposed upon engagement of the T-Cell Receptor complex. Exposure of this neoepitope precedes CD3 phosphorylation and recruitment and activation of ZAP-70, which initiates the signaling cascade produced by T-cell activation. As such, clone APA1/1 provides the earliest known marker for T-Cell Receptor engagement, and thus T-cell activation.

Detect CD3ε using this Anti-CD3ε Antibody, clone APA1/1 validated for use in FC, IP, WB & IC.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Quality

routinely evaluated by immunoblot on RIPA lysates from Jurkat cells

Target description

~23kDa

Linkage

Replaces: 04-460

Physical form

0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide

Format: Purified

Protein G Purified

Storage and Stability

1 year at 2-8°C from date of shipment.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

¿No encuentra el producto adecuado?

Pruebe nuestro Herramienta de selección de productos.

Optional

Referencia del producto

Descripción

Precios

ZRB1011

Anti-CD3e Antibody, clone 1H4, ZooMAb^® Rabbit Monoclonal, recombinant, expressed in HEK 293 cells

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

The CD3-gamma and CD3-delta subunits of the T cell antigen receptor can be expressed within distinct functional TCR/CD3 complexes.

B Alarcón et al.

The EMBO journal, 10(4), 903-912 (1991-04-01)

The T cell receptor for antigen (TCR) consists of two glycoproteins containing variable regions (TCR-alpha/beta or TCR-gamma/delta) which are expressed on the cell surface in association with at least four invariant proteins (CD3-gamma, -delta, -epsilon and -zeta). CD3-gamma and CD3-delta

Conformational and biochemical differences in the TCR.CD3 complex of CD8(+) versus CD4(+) mature lymphocytes revealed in the absence of CD3gamma.

D A Zapata et al.

The Journal of biological chemistry, 274(49), 35119-35128 (1999-11-27)

Mature CD4(+) and CD8(+) T lymphocytes are believed to build and express essentially identical surface alphabeta T-cell receptor-CD3 (TCR.CD3) complexes. However, TCR.CD3 expression has been shown to be more impaired in CD8(+) cells than in CD4(+) cells when CD3gamma is

Recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation.

Diana Gil et al.

Cell, 109(7), 901-912 (2002-07-12)

How membrane receptors initiate signal transduction upon ligand binding is a matter of intense scrutiny. The T cell receptor complex (TCR-CD3) is composed of TCR alpha/beta ligand binding subunits bound to the CD3 subunits responsible for signal transduction. Although it

Characterization of the region involved in CD3 pairwise interactions within the T cell receptor complex.

A Borroto et al.

The Journal of biological chemistry, 273(21), 12807-12816 (1998-05-28)

Assembly of the six-chain T cell antigen receptor-CD3 complex takes place by pairwise interactions. Thus, CD3-epsilon interacts with either CD3-gamma or CD3-delta, and these dimers then associate with the TCR heterodimer (alpha.beta or gamma.delta) and the CD3-zeta homodimer to constitute

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico