05-667
Anti-phospho-CREB (Ser133) Antibody, clone 10E9
clone 10E9, Upstate®, from mouse
Sinónimos:
active transcription factor CREB, cAMP responsive element binding protein 1, cAMP-response element-binding protein-1, transactivator protein
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
10E9, monoclonal
species reactivity
canine, rat, mouse, human
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pSer133)
Gene Information
human ... CREB1(1385)
General description
Cyclic AMP-responsive element-binding protein 1 (UniProt: P16220; also known as CREB-1, cAMP-responsive element-binding protein 1) is encoded by the CREB1 gene (Gene ID: 1385) in human. CREB is a phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE; TGACGTCA) as a dimer. It is essential for the activation of a number of immediate early genes. It also plays a key role in promoting neuronal survival, differentiation, proliferation, and neurite outgrowth. Transcription activation is reported to be enhanced by the TORC coactivators. CREB has four main functional domains: a C-terminal basic/leucine zipper domain (bZIP; aa 269-327), the Kinase Inducible Domain at the N-terminal (KID; aa 87-146), and two glutamine-rich domains (Q1, and Q2). The Q1 and Q2 domains provide transactivation function in the absence of cAMP signaling and synergize with KID and bZIP to confer full activity to CREB in response to cAMP. CREB is phosphorylated on serine 133 by protein kinase A, which promotes the recruitment of the co-activator CREB-binding protein(CBP) and p300 that increases the transcription of CREB-dependent genes. CREB is also phosphorylated on serine 133 by mitogen- and stress-activated kinase 1/2 (MASK 1/2) in cells in response to the activation of MAPK signaling. Phosphorylation of CREB is sufficient to induce cellular gene expression in response to cAMP, however, additional promoter-bound factors are required for target gene activation by CREB in response to mitogen or stress signals, indicating the presence of components in the CREB pathway that discriminate between different types of inputs. (Ref.: Wang, H., et al. (2018). Front. Mol. Neurosci. 11; 255; Naqvi, S., et al. (2014) Biochem. J. 458(3); 469-479; Mayr, B., and Montiminy, M. (2001). Nat. Rev. Mol. Cell Biol. 2(8); 599-609)..
Specificity
Recognizes phosphorylated CREB at Mr 46 kDa. May also recognize p30 and p38 proteins, which likely correspond to phosphorylated CREM and ATF-1 respectively, since the two proteins share significant homology with the phosphorylated CREB immunizing peptide
Immunogen
KLH-conjugated, synthetic peptide (REILSRRP(pS)YR) corresponding to amino acids 125-135 of human CREB. The immunizing sequence is identical in human and mouse. Clone 10E9.
Application
Anti-phospho-CREB (Ser133) Antibody, clone 10E9 is a Mouse Monoclonal Antibody for detection of phospho-CREB (Ser133) also known as active transcription factor CREB & has been validated in WB, ELISA.
ELISA: Recommended at 0.05 μg/mL by an independent laboratory.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Quality
Evaluated by western blot on whole cells lysates from rat PC12 cells treated with forskolin.
Western Blot Analysis: 0.5-2 μg/mL of this antibody detected phosphorylated CREB in whole cell lysates from HepG2 and PC12 cells treated with forskolin.
Western Blot Analysis: 0.5-2 μg/mL of this antibody detected phosphorylated CREB in whole cell lysates from HepG2 and PC12 cells treated with forskolin.
Target description
46 kDa
Physical form
Format: Purified
Purified mouse monoclonal IgG1κ from serum-free cell culture supernatant, lyophilized from 1 mL of PBS, 0.09% sodium azide, PEG, and sucrose. Lyophilized.
Thiophilic adsoption and size exclusion chromatography
Storage and Stability
Lyophilized: Stable for 1 year at -20ºC from date of receipt.
Rehydrated: Stable for 3 months at 2-8ºC.
Aliquot solution to store frozen and avoid repeated freeze thaw cycles.
Rehydrated: Stable for 3 months at 2-8ºC.
Aliquot solution to store frozen and avoid repeated freeze thaw cycles.
Analysis Note
Control
Forskolin-treated PC12 cell lysate
Forskolin-treated PC12 cell lysate
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
¿No encuentra el producto adecuado?
Pruebe nuestro Herramienta de selección de productos.
Optional
Referencia del producto
Descripción
Precios
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk_germany
WGK 1
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
David V C Brito et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 40(6), 1197-1210 (2019-12-13)
Age-related memory loss is observed across multiple mammalian species and preferentially affects hippocampus-dependent memory. Memory impairments are characterized by accelerated decay of spatial memories. Nevertheless, the molecular mechanisms underlying these deficits are still largely unknown. Here, we investigated the expression
Yi-Cheng Chang et al.
EMBO molecular medicine, 5(8), 1165-1179 (2013-07-06)
Elevated oxidative stress is closely associated with obesity. Emerging evidence shows that instead of being a consequence of obesity, oxidative stress may also contribute to fat formation. Nonselenocysteine-containing phospholipid hydroperoxide glutathione peroxidase (NPGPx) is a conserved oxidative stress sensor/transducer and
Laura R Pearce et al.
The Biochemical journal, 431(2), 245-255 (2010-08-14)
S6K1 (p70 ribosomal S6 kinase 1) is activated by insulin and growth factors via the PI3K (phosphoinositide 3-kinase) and mTOR (mammalian target of rapamycin) signalling pathways. S6K1 regulates numerous processes, such as protein synthesis, growth, proliferation and longevity, and its
F Spiga et al.
Journal of neuroendocrinology, 23(2), 136-142 (2010-11-19)
The availability of active steroidogenic acute regulatory protein (StAR) and side-chain cleavage cytochrome P450 (P450scc) are rate-limiting steps for steroidogenesis. Transcription of StAR and P450scc genes depends on cyclic AMP-response element-binding protein (CREB) phosphorylation and CREB co-activator, transducer of regulated
Caodu Buren et al.
Journal of neurophysiology, 116(2), 380-390 (2016-04-29)
Corticostriatal cocultures are utilized to recapitulate the cortex-striatum connection in vitro as a convenient model to investigate the development, function, and regulation of synapses formed between cortical and striatal neurons. However, optimization of this dissociated neuronal system to more closely
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico