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906476

Sigma-Aldrich

SLAM-Aβ-13CHD2 Methyl Labeling Kit

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.12

technique(s)

bio NMR: suitable

Quality Level

shipped in

dry ice

storage temp.

−70°C

General description

SLAM-Aβ-13CHD2 kit has 13CHD2 isotopomer precursor and contains protocol instructions for creation of isotopically-labeled proteins.

Application

For solid-state NMR applications and dynamic studies.
For regio- and/or stereo- specific isotope labelilng of Isoleucine, Leucine, Valine, and Alanine with 13CHD2 isotopomers.
SLAM-Aβ-13CHD2 kit is used for labeling alanine precursor with 13CHD2 isotopomer. This kit has been tested with protein isotopic labeling in E. coli. It can be employed for the study of side chain dynamics of protein in the solid-state NMR spectroscopy.

Packaging

This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

Storage Class

11 - Combustible Solids


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Maggy Hologne et al.
Journal of the American Chemical Society, 127(32), 11208-11209 (2005-08-11)
We show in this communication that dynamic information for uniformly 2H,13C,15N isotopically enriched, crystalline proteins can be obtained by MAS solid-state NMR spectroscopy. The experiments make use of the deuterium quadrupolar tensor, which is the dominant interaction mechanism. Dynamic properties
Silke Wiesner et al.
Current opinion in structural biology, 35, 60-67 (2015-09-26)
Intermolecular interactions are indispensible for biological function. Here we discuss how novel NMR techniques can provide unique insights into the assembly, dynamics and regulation of biomolecular complexes. We focus on applications that exploit the methyl TROSY effect and show that

Artículos

Methyl groups are ideal probes for studying protein structure and function by NMR. Choose among user-friendly kits for isotope labeling of a single type or multiple types of methyl groups.

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