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Key Documents

906468

Sigma-Aldrich

SLAM-VproS-13CHD2 Methyl Labeling Kit

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.12

technique(s)

bio NMR: suitable

Quality Level

200

shipped in

dry ice

storage temp.

−70°C

Categorías relacionadas

General description

SLAM-VproS-13CHD2 kit has 13CHD2 isotopomer precursor and contains protocol instructions for creation of isotopically-labeled proteins.

Application

For solid-state NMR applications and dynamic studies.
For regio- and/or stereo- specific isotope labelilng of Isoleucine, Leucine, Valine, and Alanine with 13CHD2 isotopomers.
SLAM-VproS-13CHD2 kit is used for stereospecific labeling of valine with 13CHD2 isotopomer. This kit has been tested with protein isotopic labeling in E. coli. It is beneficial for sample stability, which reduces sample heating because of high power decoupling when studying the side chain dynamics of proteins through solid-state NMR spectroscopy.

Packaging

This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

Storage Class

11 - Combustible Solids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Rime Kerfah et al.
Current opinion in structural biology, 32, 113-122 (2015-04-17)
Nuclear magnetic resonance (NMR) spectroscopy is a uniquely powerful tool for studying the structure, dynamics and interactions of biomolecules at atomic resolution. In the past 15 years, the development of new isotopic labeling strategies has opened the possibility of exploiting
Silke Wiesner et al.
Current opinion in structural biology, 35, 60-67 (2015-09-26)
Intermolecular interactions are indispensible for biological function. Here we discuss how novel NMR techniques can provide unique insights into the assembly, dynamics and regulation of biomolecular complexes. We focus on applications that exploit the methyl TROSY effect and show that

Artículos

Methyl Isotope-Labeling of Proteins from NMR-Bio

Methyl groups are ideal probes for studying protein structure and function by NMR. Choose among user-friendly kits for isotope labeling of a single type or multiple types of methyl groups.

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