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Key Documents

G005751

HPLC Analysis of Sugars and Taurine in an Energy Drink on Ascentis® Express HILIC using UV and ELSD Detection in Series

application for HPLC

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About This Item

技術

HPLC: suitable

測試參數

column: Ascentis Express HILlC, 10 cm x 3.0 mm I.D., 2.7 μm particles (53970-U)
mobile phase: [A] 100 mM ammonium acetate, pH 5.0 with acetic acid; [B] water; [C] acetonitrile; (09:01:90, A:B:C)
flow rate: 0.6 mL/min
pressure: 812 psi (56 bar)
column temp.: 35 °C
detector: ELSD, 55 °C, 3.5 bar nitrogen
injection: 2 μL
sample: dilute1:9 in acetonitrile

適合性

application for HPLC

應用

food and beverages

分析報告

This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of common drink additives used in caffeinated energy drinks. The analysis was performed with UV and ELSD detectors in series, this chromatogram being from the ELSD.

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

分析物

描述

    Sucrose
    Taurine

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