G005682

HPLC Analysis of Caffeine and Taurine on Ascentis^® Express HILIC Column using Evaporative Light Scattering Detection (ELSD)

application for HPLC

屬性

• 技術: HPLC: suitable
• 測試參數: column: Ascentis Express HILlC, 5 cm x 3.0 mm I.D., 2.7 μm particles (53967-U); mobile phase: [A] 100 mM ammonium formate, pH 3.0 with formic acid; [B] acetonitrile; (10:90, A:B); flow rate: 0.6 mL/min; column temp.: 25 °C; detector: ELSD, 55 °C, 3.5 bar nitrogen; injection: 2 μL; sample: 1000 μg/mL in 10:90, water: acetonitrile
• 適合性: application for HPLC
• 應用: vitamins, nutraceuticals, and natural products

描述

分析報告

This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of caffeine and taurine. Elution is by aqueous normal phase (ANP) as an increase in organic will increase retention.

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

分析物

Caffeine
Taurine