技術
HPLC: suitable
測試參數
column: Ascentis RP-Amide, 15 cm x 2.1 mm I.D., 5 μm particles (565305-U)
mobile phase: [A] 0.1% ammonium acetate in water (pH unadjusted); [B] acetonitrile
gradient: 25 to 75% B in 10 min; held at 75% B for 5 min
flow rate: 0.5 mL/min
column temp.: 35 °C
detector: UV, 340 nm
injection: 10 μL
適合性
application for HPLC
應用
clinical
分析報告
Following pre-column derivatization of primary amino groups using o-phthaldialdehyde/thiol reagent, the Ascentis RP-Amide was used for the analysis of biogenic amines commonly found in wines. These include histamine, spermine, spermidine, tyramine, putrescine and cadaverine. Structures along with an optimized chromatogram are presented.
法律資訊
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
描述
Histamine
Spermine
Spermidine
Tyramine
1,4-Diaminobutane
Cadaverine
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