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長度 × 內徑
5 cm × 5 mm
基質
MonoBeads
基質活性組
amphoteric
粒徑
10 μm
相容性
mode of use ion exchange
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一般說明
Mono 色谱柱是高效、pH 稳定的色谱柱，设计用于蛋白质、多肽和多核苷酸的高效离子交换分离，应用范围包括肽图谱和单克隆抗体纯化。这些色谱柱的独特属性是基于单柱支撑-一种珠状亲水性材料，具有任何色谱支撑体中最窄的粒度分布。这种单分散性允许在相对较低的反向压力下获得较高的流速。
應用
Mono columns are used in peptide mapping and monoclonal antibody purification. Mono P columns have been used to purify homogenous forms of fungal peroxygenase and to investigate separation of DNA restriction fragments.
法律資訊
Mono P is a registered trademark of Cytiva
René Ullrich et al.
Biotechnology journal, 4(11), 1619-1626 (2009-07-09)
Extracellular peroxygenase from the agaric fungus Agrocybe aegerita is a versatile biocatalyst that oxygenates various substrates by means of hydrogen peroxide. The enzyme is routinely produced in suspensions of soybean meal and has until now been purified by several steps
E Westman et al.
Analytical biochemistry, 166(1), 158-171 (1987-10-01)
Separation of DNA restriction fragments by FPLC ion-exchange chromatography on Mono Q and Mono P columns was investigated. The columns were found to be particularly suitable for the separation of fragments up to 500-600 bp long. Larger fragments can also
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