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product name
BIOshell Glycan HPLC Columns, L × I.D. 15 cm × 2.1 mm, 2.7 μm particle size
材料
stainless steel hardware (column)
品質等級
agency
suitable for USP L86
描述
shell thickness (0.5 μm)
solid core (1.7 μm)
產品線
BIOshell
特點
endcapped: no
分析物化學類別
glycans
製造商/商標名
BIOshell
包裝
1 ea of
參數
1000 bar max. pressure (14500 psi)
1000 bar pressure (14500 psi)
60 °C max. temp.
技術
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
長度 × 內徑
15 cm × 2.1 mm
表面積
135 m2/g
基質
spherical silica particle platform
superficially porous particle
基質活性組
hydroxy, penta- phase
粒徑
2.7 μm
孔徑
90 Å
工作pH值範圍
2-9
分離技術
hydrophilic interaction (HILIC)
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一般說明
View the BIOshell Glycan HPLC Columns Flyer
應用
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分析證明 (COA)
文章
The BIOshell™ Glycan column is ideal for the separation and quantitation of isolated glycans using the HILIC method for accurate glycoprotein analysis.
The BIOshell™ Glycan column is ideal for the separation and quantitation of isolated glycans using the HILIC method for accurate glycoprotein analysis.
The use of PNGase Fast denaturing buffer and enzyme yielded results similar to a conventional 20-hour protocol with overnight digest while reducing workflow time to about 1 hour with a 15-minute digest.
相關內容
HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.
High performance liquid chromatography (HPLC) can be used to separate and identify different large biomolecules such as protein and peptides in a sample. It is based on the pumping of a sample with a solvent (mobile phase) through a column packed with sorbent material (stationary phase) at a high pressure.
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