ZRB06182

Anti-p44 MAPK (ERK1) Antibody, clone 13F10, ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: EC: 2.7.11.24, ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, MNK1, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 3, p44-ERK1
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit (recombinant)
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 13F10, recombinant monoclonal; monoclonal
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 43.08 kDa; observed mol wt ~44 kDa
• 物種活性: mouse, rat, human
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using 1:100; immunoprecipitation (IP): suitable using 1:100; western blot: suitable using 1:1,000
• 同型: IgG
• UniProt登錄號: P21708
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: rat ... Mapk3;Erk1;Prkm3(50689)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特異性

Clone 13F10 is a ZooMAb rabbit recombinant monoclonal antibody that detects p44 MAPK (ERK1). May also display reactivity with p42 MAPK (ERK2).

免疫原

KLH-conjugated linear peptide corresponding to 35 amino acids from the C-terminal region of rat p44 MAPK (ERK1).

應用

Anti-p44 MAPK (ERK1), clone 13F10 ZooMAb, Cat. No. ZRB06182, is a recombinant Rabbit Monoclonal Antibody that detects p44 MAPK (ERK1) and has been tested for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Western Blotting Analysis: 1:1,000 dilution from a representative lot detected p44 MAPK (ERK1) in Rat C6, L6, and mouse NIH/3T3 cell lysates.

Immunoprecipitation Analysis: 100 μL from a representative lot immunoprecipitated p44 MAPK (ERK1) in Rat L6 cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected p44 MAPK (ERK1) in rat colon and rat cerebral cortex tissue sections.

標靶描述

Mitogen-activated protein kinase 3 (UniProt: P21708; also known as EC: 2.7.11.24, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, MNK1, Microtubule-associated protein 2 kinase, p44-ERK1) is encoded by the Mapk3 (also known as Erk1, Prkm3) gene (Gene ID: 50689) in rat. ERK-1 is a serine/threonine kinase that acts as an essential component of the MAP kinase signal transduction pathway. It is expressed in different tissues, mostly in intestine, placenta, and lung. Highest levels have been reported in the nervous system. The MAPK/ERK cascade mediates several diverse biological processes such as cell growth, adhesion, survival, and differentiation through the regulation of transcription, translation, and cytoskeletal rearrangements. It also plays also a role in initiation and regulation of meiosis, mitosis, and post-mitotic functions in differentiated cells by phosphorylating a few transcription factors. About 160 substrates have been described for ERKs. The protein kinase domain of ERK-1 is localized to amino acids 43-331 and the TXY motif is located in amino acids 203-205. ERK-1 is phosphorylated by MEK1 and MEK2 on threonine 203 and tyrosine 205 in response to external stimuli like insulin or nerve growth factor and both these phosphorylations are essential for its activity. Two isoforms of ERK-1 have been described that are produced by alternative splicing. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable