ZMS1130

Anti-GABRB2 Antibody, clone BD17 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: Gamma-aminobutyric acid receptor subunit beta-2;GABA(A) receptor subunit beta-2
• 分類程式碼代碼: 12352203

屬性

• 生物源: mouse
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: BD17, recombinant monoclonal
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 54.45 kDa; observed mol wt ~52 kDa
• 純化經由: using protein G
• 物種活性: human, mouse
• 物種活性（以同源性預測）: bovine, feline, rat, monkey
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• 技術: immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG1κ
• 表位序列: Unknown
• Protein ID登錄號: NM_000813.2
• UniProt登錄號: P0C2W5
• 環保替代類別: Aligned,
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: bovine ... GABRB2(540657)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone BD17 is a ZooMAb^® Mouse recombinant monoclonal antibody that specifically detects GABA A receptor β2.

免疫原

Purified GABA A receptor from bovine cerebral cortex.

應用

Quality Control Testing

Evaluated by Western Blotting in Mouse liver membrane preparation.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected GABRB2 in Mouse liver membrane preparation.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GABRB2 in Human brain and Mouse brain tissue sections.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected GABRB2 in Human cerebellum tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

Gamma-aminobutyric acid receptor subunit beta-2 (UniProt: P0C2W5; also known as GABA(A) receptor subunit beta-2) is encoded by the GABRB2 gene (Gene ID: 540657) in Bovine. GABA is the major inhibitory neurotransmitter in the central nervous system that inhibits the generation of the action potential of the neuron. It plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel. It is involved in the pathogenesis of certain neurological and psychiatric disorders. GABA interacts with the GABAA and GABAB receptors, which are widely distributed throughout the nervous system and are involved in synaptogenesis, neurogenesis, and the regulation of neuronal plasticity and learning. These receptors differ in their pharmacological, electrophysiological, and biochemical properties. GABRB2 is a multi-pass membrane protein that plays an important role in the formation of the pentameric structure of the GABA A receptor. It is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 24-239), three transmembrane domains, and a cytoplasmic domain (aa 330-450). The extracellular domains contribute to synaptic contact formation. GABRB2 is highly expressed in the brain with abundance in the gray matter of neurons and glial cells. It is associated with cognitive function related to time perception. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Barki, M., and Xue, H. (2022). Gene. 809;146021; Ymer, S., et al. (1989). EMBO J. 8(6); 1665-1670).

外觀

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

重構

0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable