ZMS1054

Anti-Centrin Antibody, clone 20H5 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 20H5, recombinant monoclonal; monoclonal
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt ~19.46 kDa; observed mol wt ~18 kDa
• 物種活性: human, mouse
• 物種活性（以同源性預測）: Chlamydomonas reinhardtii, rat
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 同型: IgG2a
• UniProt登錄號: P05434
• 環保替代類別: Aligned,
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

特異性

Clone 20H5 is a ZooMAb^® mouse recombinant monoclonal antibody that specifically detects centrin (Caltractin). It targets an epitope within the C-terminal region.

免疫原

Recombinant Chalamydomonas reinhardtii centrin.

應用

Quality Control Testing

Evaluated by Western Blotting in Mouse testis tissue lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Centrin in Mouse testis tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Centrin in human testis tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Centrin in human testis tissue sections.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Centrin in human testis tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Centrin, clone 20H5 ZooMAb^®, Cat. No. ZMS1054, is a recombinant Mouse monoclonal antibody that specifically targets Centrin and is tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

標靶描述

Caltractin (UniProt: P05434; also known as 20 kDa calcium-binding protein, Centrin) is encoded by the VFL2 gene (Gene ID: 5725094) in Chlamydomonas reinhardtii (algae). Centrin is a highly conserved calmodulin-like calcium-binding phospho-protein that contain four EF hands and are associated with centrosome-related structures. In Chlamydomonas reinhardtii, centrin is shown to play an essential role in microtubule severing during flagellar excision during which time stellate fibers of the transition zone contract and displace the microtubule doublets of the axoneme inward. The resulting shear force and torsional load generated during inward displacement leads to microtubule severing immediately distal to the central cylinder of the transition zone. Centrin is also present is all eukaryotic cells and contain four helix-loop-helix motifs that all conform to the EF-hand consensus for Ca2+ binding. Two of these motifs exhibit high affinity for Ca2+ two display lower affinity. Stability of centrin is regulated by phosphorylation of serine residue in the C-terminal region by aurora kinase A, which also localizes with centrin at the centrosome. Their levels are shown to be the highest from prophase to metaphase stage followed by precipitous loss in the late stage of mitosis. In human breast cancers higher levels of aurora kinase A and phospho-centrin have been observed. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Sanders, MA., and Salisbury, JL. (1994). J. Cell Biol. 24(5); 795-805; Lutz, W., et al. (2001). J. Biol. Chem. 276(23); 20774-20780; Lukasiewicz, KB., et al. (2011). PLoS ONE 6(6); e21291).

外觀

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1