VRE100

Viral RNA Extraction Buffer

One Step RT-qPCR Compatible, Optimized for Viral RNA Detection in Saliva

• 同義詞: For use with Reverse Transcription-qPCR, Viral Lysis Solution for RT-qPCR, Viral RNA Extraction
• 分類程式碼代碼: 12161703
• NACRES: NA.52

屬性

• 等級: Molecular Biology
• 品質等級: 200
• 形狀: liquid
• 濃度: 4X
• 技術: LAMP: suitable; PCR: suitable; RT-PCR: suitable; qPCR: suitable
• 運輸包裝: wet ice
• 儲存溫度: −20°C

描述

一般說明

The Viral RNA Extraction Buffer has been optimized to provide rapid, room-temperature lysis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral particles in saliva. The buffer comes as a 4x working solution that can be mixed with virus-containing samples in a qRT-PCR plate. Upon 5 minute incubation at room temperature, viral particles are lysed and RNA is stabilized such that samples are ready for direct analysis via RT-qPCR with no additional cleanup step. Removal of precipitates in saliva via centrifugation prior to testing is recommended for optimal sensitivity.

應用

The Viral RNA Extraction Buffer is broadly compatible with most nucleic acid detection methods and does not reduce signal when used at working concentration in qRT-PCR. qRT-PCR compatibility has been demonstrated in a 4-fluor multiplex setting consisting of Cy5, TxRed, FAM, and HEX.

RT-qPCR compatibility has been verified with several one-step RT-qPCR kits:

• Quantitative RT-PCR ReadyMix^™ (Sigma-Aldrich, catalog QR0200)
• KAPA PROBE FAST One-Step Universal (Sigma-Aldrich, catalog KK4752)
• KiCqStart^® One-Step Probe RT-qPCR ReadyMix^™ (Sigma-Aldrich, catalog KCQS07)
• TaqPathTM 1-Step Multiplex Master Mix (Thermo Fisher, catalog A28525)

The Viral RNA Extraction Buffer is also compatible with colorimetric LAMP assays.

特點和優勢

• Extracted viral RNA can be used for detection without cleanup
• Directly compatible with quantitative reverse transcription polymerase chain reaction (qRT-PCR) and loop mediated isothermal amplification(LAMP) assays
• Ideal for enveloped RNA viruses in saliva, saline, Amies medium, and viral transport medium
• Highly sensitive and faster than other leading methods, lysis by Proteinase K and heating

法律資訊

KiCqStart is a registered trademark of QIAGEN Beverly Inc.

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

安全資訊

• 象形圖: GHS05
• 訊號詞: Danger
• 危險聲明: H318
• 防範說明: P280 - P305 + P351 + P338
• 危險分類: Eye Dam. 1
• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable