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一般說明
冻干粉末,含有蔗糖、氯化钠和Tris。
凝血酶是止血过程中的最终凝血蛋白酶,可同时促进促凝血和抗凝血作用。
應用
凝血酶被用于重组融合蛋白的位点特异性切割,这些重组融合蛋白具有可接近的凝血酶识别位点以除去亲和标签。凝血酶已应用于一项研究,以评估用于小尺寸血小板微粒的流式细胞术的创新方法。
生化/生理作用
选择性裂解纤维蛋白原中 Arg-Gly 键形成纤维蛋白和纤维蛋白肽 A 和 B 的丝氨酸蛋白酶。
單位定義
活性以NIH单位表示,它是通过与NIH凝血酶参考标准品直接对比而获得的
分析報告
NIH测定程序根据Biggs改进方法,使用0.2ml稀释血浆(与生理盐水1:1)作为底物以及0.1ml凝血酶样本(在1%缓冲白蛋白溶液中稳定)。仅在15-25 秒的凝血时间内测定凝血酶浓度。
其他說明
请访问www.sigma-aldrich.com/enzymeexplorer查看有关凝血酶的更多信息。
免責聲明
仅供研究使用。本品在法国的科研应用受到监管,包括用于进口和出口活动(法国《公共卫生法典》Public Health Code Article L 1211-1 paragraph 2)。如《公共卫生法典》所述,购买者(即最终用户)需要从法国研究部(France Ministry of Research)获得进口授权。订购此产品即代表您已确认获得合法的进口授权。
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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Thrombin Factor IIa is an endolytic serine protease that selectively cleaves the Arg--Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.
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