T5327

Anti-TTP (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• 同義詞: Anti-G0/G1 switch regulatory protein 24, Anti-GOS24, Anti-Growth factor-inducible nuclear protein NUP475, Anti-NUP475, Anti-RNF162A, Anti-TIS11, Anti-Tristetraprolin, Anti-Zfp36, Zinc finger protein 36 homolog Protein
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~50 kDa
• 物種活性: human
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• 濃度: ~1.0 mg/mL
• 技術: immunoprecipitation (IP): 5-10 μg/mL using lysates of RAW264 cells induced with 10 ng/mL LPS for two hours; western blot: 0.5-1.0 μg/mL using lysates of RAW264 cells induced with 10 ng/mL LPS for two hours
• UniProt登錄號: P26651
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ZFP36(7538) ; mouse ... Zfp36(22695) ; rat ... Zfp36(79426)

描述

一般說明

TTP belongs to a family of human ARE (AU-rich element found in 3′ UTR mRNA) binding protein TTP mRNA is widely distributed among tissue types, with a higher expression level in spleen, lymph nodes, and thymus.

Tristetraprolin (TTP), also known as zinc-finger protein 36 (ZFP36), is a RNA-binding protein, encoded by the gene mapped to human chromosome 19q13.2. TTP is ubiquitously expressed and is characterized with a highly homologous RNA binding domain (RBD) with two CCCH zinc fingers (ZFs).

免疫原

synthetic peptide corresponding to amino acids 51-67 of human TTP, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat.

應用

TTP (N-terminal) antibody produced in rabbit has been used in following studies:

• RNA EMSA supershift
• immunoblotting
• ribonucleoprotein immunoprecipitation (RNP-IP)

生化／生理作用

In activated monocytes and T lymphocytes, it regulates the expression of tumor necrosis factor a (TNF- α) by binding to a conserved AU rich element within the 3′ UTR of TNF-α mRNA. TTP is a phosphoprotein reported to be a substrate for kinases like MAPK activated protein kinase 2 (MAPKAPK2). The p38 signal transduction pathway is required for the expression of TTP protein and mRNA.

Tristetraprolin (TTP) plays a key role in maintaining the stability of certain adenosine/uridine AU-rich element (ARE) mRNAs, by removing of the poly(A) tail and enhancing mRNA turnover. The encoded protein participates in mRNA binding and destabilization.

標靶描述

TTP (N-terminal) (also known as Tristetraproline, Zfp-36, TIS11A, and Growth factor-inducible nuclear protein NUP475) is an RNA-binding protein that suppresses inflammation by accelerating the degradation of cytokine mRNA. TTP belongs to a family of human

外觀

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)