T2824

Monoclonal Anti-Tumor Necrosis Factor-α antibody produced in hamster

~2 mg/mL, clone TN3-19.12, purified immunoglobulin, buffered aqueous solution

• 同義詞: Anti-TNF-α
• MDL號碼: MFCD01632994
• 分類程式碼代碼: 51111800
• NACRES: NA.41

屬性

• 生物源: hamster
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: TN3-19.12, monoclonal
• 形狀: buffered aqueous solution
• 物種活性: rat, mouse
• 包裝: antibody small pack of 25 μL
• 濃度: ~2 mg/mL
• 技術: immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; neutralization: suitable; western blot: 0.25-0.5 μg/mL using recombinant mouse TNF-α
• UniProt登錄號: P16599
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... Tnf(21926) ; rat ... Tnf(24835)

描述

一般說明

Monoclonal Anti-Mouse Tumor Necrosis Factor-α (TNF-α) is derived from the TN3-19.12 hybridoma produced by the fusion of mouse myeloma cells (P3X63-Ag8.653) and splenocytes from male Armenian hamster immunized with purified mouse recombinant TNF-α. Tumor necrosis factor (TNF) protein family contains two members: TNF-α (also known as cachectin) and TNF-β (also known as lymphotoxin). These proteins are small molecules with a molecular weight of 17 and 25 kDa respectively and share 30% amino acid homology. TNF-α and -β are secreted from many different cells such as lymphocytes, neutrophils, and macrophages.

特異性

Mouse monoclonal clone TN3-19.12 anti-Tumor Necrosis Factor-α antibody recognizes mouse and rat TNF-α (approx. 17 kDa in its monomer form and 51 kDa in its homotrimer form) and cross-reacts with mouse TNF-β.

免疫原

purified recombinant mouse TNF-α.

應用

Monoclonal Anti-Tumor Necrosis Factor-α antibody produced in hamster has been used in:

• immunoblotting
• enzyme-linked immunosorbent assay (ELISA)
• immunoprecipitation
• neutralization

生化／生理作用

TNF-α is a major mediator of inflammatory responses and its over-production results in extensive tissue damage associated with endotoxemia and cachexia. High serum levels of TNF are associated with septic shock, rejection of renal transplants, parasitic infections, and various neoplastic diseases. TNF-α exhibits growth-enhancing activities for normal cells like fibroblasts, T and B cells, activation of endothelial cells, and differentiation of myeloid cells. In neoplastic cells, TNF-α exerts growth inhibitory activities that can be either cytostatic or cytotoxic.

外觀

Supplied as a 0.2 μm filtered solution in 0.01 M phosphate buffered saline, pH 7.4.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable