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![N-[3-(2-呋喃基)丙烯酰基]-亮氨酸-甘氨酸-脯氨酸-丙氨酸](/deepweb/assets/sigmaaldrich/product/structures/805/876/96b5fb57-71c8-4c6b-b5d2-fafe7374cd85/640/96b5fb57-71c8-4c6b-b5d2-fafe7374cd85.png)
品質等級
化驗
≥99% (titration)
形狀
crystalline powder
有用的pH值範圍
7.4-8.8
pKa (25 °C)
8.1
mp
187 °C
溶解度
water: 0.25 g/mL, clear, colorless
應用
diagnostic assay manufacturing
SMILES 字串
OCC(CO)(CO)NCC(O)=O
InChI
1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
InChI 密鑰
SEQKRHFRPICQDD-UHFFFAOYSA-N
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一般說明
Tricine 是最常用的电泳缓冲液，也可用于细胞颗粒重悬。与甘氨酸-十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）系统相比，在丙烯酰胺浓度较低的情况下，Tricine 起到尾随离子的作用，有助于小蛋白质的分离。它的负电荷比甘氨酸低，这有助于它更快地迁移。Tricine 具有高离子强度，可以增加离子运动，减少蛋白质运动。这反过来又导致低分子量蛋白质在较低百分比的丙烯酰胺凝胶中分离。
應用
Tricine 已用于：
- 防止赫氏颗石藻培养物高压灭菌过程中的盐沉淀
- 作为缓冲液 A 组分，用于均质化秀丽隐杆线虫、果蝇和植物等样品
- 作为新鲜检测缓冲液成分，放射免疫法测定血清褪黑素
用于分离低分子量的肽的缓冲剂组分。
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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條款
This procedure may be used for Collagenase products.
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