T17917

三(羟甲基)甲基甘氨酸

≥99% (titration), reagent grade, crystalline

• 同義詞: N-三(羟甲基)甲基甘氨酸
• 線性公式: (HOCH₂)₃CNHCH₂CO₂H
• CAS號碼: 5704-04-1
• 分子量：: 179.17
• Beilstein: 1937804
• MDL號碼: MFCD00004277
• 分類程式碼代碼: 12161700
• NACRES: NA.25

屬性

• 生物源: synthetic
• 品質等級: 200
• 等級: reagent grade
• 化驗: ≥99% (titration)
• 形狀: crystalline
• 儲存條件: (Tightly closed. Dry)
• 雜質: <0.5% water
• 顏色: white
• 有用的pH值範圍: 7.4-8.8
• pKa (25 °C): 8.1
• mp: 187 °C
• 溶解度: water: 0.1 M, clear, colorless
• 正離子痕跡: Pb: ≤5 ppm
• 儲存溫度: room temp
• SMILES 字串: OCC(CO)(CO)NCC(O)=O
• InChI: 1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
• InChI 密鑰: SEQKRHFRPICQDD-UHFFFAOYSA-N

描述

一般說明

Tricine, a zwitterionic biological buffer, is commonly employed in research applications such as enzyme assays, electrophoresis, cell biology, and biochemical studies. Its increased negative charge compared to glycine ensures rapid migration during electrophoresis, facilitating efficient separation of low-molecular-weight proteins in gels with lower acrylamide percentages. Moreover, Tricine may also be used in cryopreservation media, aiding in the preservation of tissues and organs.

Tricine′s valuable properties for biological research include its zwitterionic nature, wide buffering range (pH 7.4-8.8), classification as a Good′s buffering agent, non-toxicity to cells, and high water solubility and clarity. These attributes make it suitable for metal-sensitive systems, stable pH maintenance, optimal biological applications, cell biology studies, and accurate measurements. Additionally, Tricine may find utilization in capillary zone electrophoresis, high-performance liquid chromatography, and ion exchange chromatography, demonstrating adaptability across analytical techniques.

應用

Tricine is intended to use for:

• Electrophoresis To separate low-weight peptides and proteins
• Crystallization of proteins of varying pH
• Resuspension of cell pellets
• Enzyme assays
• Tissue culture

用于分离低分子量的肽的缓冲剂组分。

特點和優勢

• Ideal as a Biological buffer for Cell Biology and Biochemical research
• Effective Buffering from pH 7.4 - 8.8 with a pKa of 8.1 (25 °C)

其他說明

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安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable