SRP6274

Furin human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• 同義詞: FUR, FURIN, PACE, PCSK3, SPC1
• 分類程式碼代碼: 12352204
• NACRES: NA.32

屬性

• 生物源: human
• 重組細胞: expressed in HEK 293 cells
• 籤條: 6-His tagged (C-terminus)
• 化驗: ≥95% (SDS-PAGE)
• 形狀: lyophilized powder
• 分子量: calculated mol wt 66.5 kDa; observed mol wt 55-66 kDa (DTT-reduced. Asp 108 is the predicted N-terminus .)
• 包裝: pkg of 10 μg
• 雜質: <1 EU/μg endotoxin (LAL test)
• UniProt登錄號: P09958
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 基因資訊: human ... PACE(5045)

描述

一般說明

Furin also known as paired basic Amino acid Cleaving Enzyme (PACE), is an enzyme which belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. The expression of furin in T-cells is required for maintenance of peripheral immune tolerance. Furin cleaves proteins just downstream of a basic amino acid target sequence (canonically, Arg-X-(Arg/Lys) -Arg′). PACE is a calcium-dependent serine endoprotease that can efficiently cleave precursor proteins at their paired basic amino acid processing sites. In addition to processing cellular precursor proteins, furin is also utilized by a number of pathogens. For example, the envelope proteins of viruses such as HIV, influenza and dengue fever viruses must be cleaved by furin or furin-like proteases to become fully functional. PACE also play a role in tumor progression.

外觀

Lyophilized from 0.22 μm filtered solution in MES, Brij-35. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

重構

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable