SRP5172

Catenin β, GST tagged human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• 同義詞: CTNNB, FLJ25606
• CAS號碼: 847964-78-7
• 分類程式碼代碼: 12352200
• NACRES: NA.32

屬性

• 生物源: human
• 重組細胞: expressed in baculovirus infected Sf9 cells
• 化驗: ≥70% (SDS-PAGE)
• 形狀: buffered aqueous glycerol solution
• 分子量: ~115 kDa
• 技術: activity assay: suitable
• 溶解度: water: soluble
• 適合性: suitable for molecular biology
• NCBI登錄號: NM_001904
• 應用: cell analysis
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... CTNNB1(1499)

描述

一般說明

Research area: Apoptosis. β-catenins are cytoplasmic proteins that are ubiquitously expressed. These proteins are associated with E-cadherin at cellular junctions. CTNNB1 (catenin β-1) belongs to the armadillo family of proteins. This protein possesses a 130 amino acid N-terminal domain, 12 imperfect repeats of 42 amino acids termed arm repeats, and a 100 amino acid C-terminal domain. The gene encoding β-catenin is localized on human chromosome 3p22.1.

應用

Catenin β, GST tagged human has been used:

• as a component of protein kinase buffer for in vitro kinase assay
• in in vitro citrullination assay to characterize β-catenin citrullination
• to perform differentiation factor screening

生化／生理作用

β-catenin interacts with T-cell factor and lymphoid enhancer-binding factor (TCF and LEF) transcription factors and is an essential member of the Wingless-Wnt signal transduction pathway. The adenomatous polyposis coli (APC) tumor-suppressor protein, together with Axin and glycogen synthase kinase 3 β (GSK3β), form a Wnt-regulated signaling complex that mediates phosphorylation-dependent degradation of βcatenin by the proteasome. APC and the Siah-1 E3 ubiquitin-protein ligase-1 mediate a novel β-catenin degradation pathway linking p53 activation to cell cycle control. Activating mutations in the human β-catenin gene have been found in human colon cancer and melanomas. The protein has a role in homeostasis and development.

外觀

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

準備報告

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable