SRP4902

Adiponectin from mouse

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• 同義詞: ACDC, APM-1, Acrp30, AdipoQ, GBP-28
• 分類程式碼代碼: 12352202
• NACRES: NA.32

屬性

• 生物源: mouse
• 重組細胞: expressed in E. coli
• 化驗: ≥90% (SDS-PAGE)
• 形狀: lyophilized
• 分子量: ~30 kDa
• 包裝: pkg of 25 μg
• 儲存條件: avoid repeated freeze/thaw cycles
• 雜質: endotoxin, tested
• NCBI登錄號: Q60994
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 基因資訊: mouse ... Adipoq(11450)

描述

一般說明

Acrp30 (adipocyte complement-related protein), or cytokine adiponectin, is a hormone produced and secreted by differentiated adipocytes. This protein is composed of an N-terminal collagenous domain and a C-terminal globular trimerization domain. It exists in plasma predominantly as trimeric, hexameric or other high-molecular weight forms. The globular subunit gAcrp30 is however absent in plasma and is biologically active. gAcrp30 is the truncated form of full length of Acrp30, and functions as a ligand of AdipoR1.

應用

Adiponectin from mouse has been used for the treatment of 3T3-L1 cells, along with TNFα (tumor necrosis factor), to determine TNFα inhibition of adiponectin activities by examining lipid accumulation and glucose uptake. It has also been used for the pretreatment of TM3 cells, to study its influence on attenuation of proinflammatory cytokine expression in TM3 cells upon treatment with TNF-α or IL-1β (interleukin).

生化／生理作用

In obese mice and humans, adiponectin mRNA expression is reduced, therefore linking this hormone to energy homeostasis. In incubated mouse muscle and cultured cells, gAcrp30 leads to an increase in fatty acid oxidation. This hormone is also linked to anti-atherogenesis, and in apoE-deficient mice, overexpression of gAcrp30 results in significant attenuation of atherosclerosis. Long-term administration of this hormone in mice results in weight loss without reduction in food intake. This hormone is capable of reducing proliferation of MDA-ERα7 cell line. Studies show that ratio of gAcrp30 to leptin and the ERα(estrogen receptor alpha) status of cells are key in determining BRCA (breast cancer) cell growth.

外觀

Lyophilized from a 0.2 μm filtered solution in 25 mM Tris, 0.15 M NaCl, pH 7.4 containing 50 μg of bovine serum albumin (BSA) per 1 μg of adiponectin.

重構

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Recontitute in PBS containing at least 0.1% BSA to a concentration of 0.1-1.0 mg/mL. This stock solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable