SRP3323

R-SPONDIN-3 human

recombinant, expressed in CHO cells, ≥95% (SDS-PAGE), ≥95% (HPLC)

• 同義詞: PWTSR, RSPO3, Roof plate-specific spondin-3
• 分類程式碼代碼: 12352202
• NACRES: NA.32

屬性

• 生物源: human
• 重組細胞: expressed in CHO cells
• 化驗: ≥95% (HPLC); ≥95% (SDS-PAGE)
• 形狀: lyophilized
• 分子量: 26.9 kDa
• 包裝: pkg of 20 μg
• 雜質: endotoxin, tested
• UniProt登錄號: Q9BXY4
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 基因資訊: human ... RSPO3(84870)

描述

一般說明

The R-Spondin (Rspo) proteins belong to the Rspo family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing furin-like and thrombospondin structural domains. The Rspo proteins can interact with the Frizzled/LRP6 receptor complex in a manner that causes the stabilization and resulting accumulation of the intracellular signaling protein, ?-catenin. This activity effectively activates and increases the subsequent nuclear signaling of ?-catenin. R-Spondin can also bind to the previously discovered G-protein coupled receptors, LGR-4 and LGR-5. Rspo/?-catenin signaling can act as an inducer of the transformed phenotype, and can also regulate the proliferation and differentiation of certain stem cell populations. Recombinant human R-Spondin-3 is a 26.9 kDa protein consisting of 240 amino acid residues. Due to glycosylation, R-Spondin-3 migrates at an apparent molecular weight of approximately 37.0 kDa by SDS PAGE analysis under reducing conditions.

生化／生理作用

The R-Spondin (Rspo) proteins belong to the Rspo family of Wnt modulators. Currently, the family consists of four structurally related secreted ligands (Rspo 1-4), all containing furin-like and thrombospondin structural domains. Recombinant human R-Spondin-3 is a 26.9 kDa protein consisting of 240 amino acid residues.

外觀

Lyophilized from 10 mM Sodium Phosphate, pH 7.5 + 150 mM NaCl.

重構

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable