SRP0721

AKT1 (E17K), Active

PRECISIO^® Kinase, recombinant

• 分類程式碼代碼: 12352202
• NACRES: NA.77

屬性

• 重組細胞: expressed in baculovirus infected Sf9 cells
• 化驗: 70-95% (SDS-PAGE)
• 形狀: frozen liquid
• 比活性: 50-75 nmol/min·mg (radiometric)
• 分子量: ~85 kDa
• 濃度: 0.1 μg/μL
• 登錄號: NM_005163
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... AKT1(207)

描述

應用

Useful for kinase assay.

生化／生理作用

Recombinant full-length human AKT1 (E17K) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. AKT1/PKBalpha is a serine/threonine kinase that belongs to the AKT family. AKT1 is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility ⁽¹⁾. AKT1 is frequently overexpressed and active in many types of human cancers including cancers of colon, breast, brain, pancreas and prostate as well as lymphomas and leukemias ⁽²⁾.

重構

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

儲存和穩定性

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

法律資訊

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable