SRP0262

PDE4A1A Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

• 同義詞: DPDE2, PDE46, PDE4A, Phosphodiesterase 4A1A
• 分類程式碼代碼: 12352200
• NACRES: NA.32

屬性

• 生物源: human
• 重組細胞: expressed in baculovirus infected insect cells
• 化驗: ≥70% (SDS-PAGE)
• 形狀: aqueous solution
• 分子量: 100 kDa
• 包裝: pkg of 10 μg
• 濃度: >0.02 mg/mL
• NCBI登錄號: U97584
• UniProt登錄號: P27815
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... PDE4A(5141)

描述

一般說明

Human PDE4A1A (GenBank Accession No. U97584), full length with N-terminal GST tag, MW = 100 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

應用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

單位定義

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C

外觀

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

準備報告

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1