SRP0221

PTPIA2 Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• 同義詞: ICA512, Islet cell antigen 2, PTPRN, Protein tyrosine phosphatase-like N, R-PTP-N
• 分類程式碼代碼: 12352200
• NACRES: NA.32

屬性

• 生物源: human
• 重組細胞: expressed in E. coli
• 化驗: ≥90% (SDS-PAGE)
• 形狀: aqueous solution
• 分子量: 58.8 kDa
• 包裝: pkg of 20 μg
• 儲存條件: avoid repeated freeze/thaw cycles
• 濃度: >0.02 mg/mL
• NCBI登錄號: NM_002846
• UniProt登錄號: Q16849
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... PTPRN(5798)

描述

一般說明

PTPRN (protein tyrosine phosphatase, receptor type N), more commonly known as IA-2 (insulinoma-associated protein 2), is a transmembrane protein of the PTP family. It was initially identified as a major autoantigen in type-1 diabetes mellitus. In neuroendocrine cells, it resides in the secretory granules. It is a multi-domain protein with intracellular, transmembrane and extracellular domains, and its intracellular region contains a pseudocatalytic domain. It gets processed multiple times during its life cycle, and its different parts are exposed to different cellular and tissue regions such as nucleus, cytosol, organellar lumen in the secretory pathway, extracellular pancreatic islet space, and membrane compartments. This gene is localized to human chromosome 2q35, and codes for a protein of 979 residues.

Protein tyrosine phosphatase. (PTP-IA2, ICA-512), Catalytic domain, (amino acids 693- 979), (Gene bank accession no. NM_002846), with N-terminal GST tag, MW = 58.8 kDa, expressed in an E. coli expression system.

應用

Useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.

生化／生理作用

PTPRN (protein tyrosine phosphatase, receptor type N) is cleaved at the cytosolic domain during insulin secretion. The cytosolic domain is relocated to nucleus where it promotes insulin gene transcription. This protein plays important role in hormone and neuropeptide secretion. This protein is crucial in controlling the dense core vesicle number in β cells and glucose-induced and basal insulin release. PTPRN null mice show reduced dense core vesicle (DCV) number, abnormal insulin secretion, female infertility, behavioral alteration, and circadian dysregulation.

單位定義

One unit will hydrolyze 1 pmol 6, 8-difluoro-4-methyl umbelliferyl phosphate (DiFMUP) per minute at pH 6.3 and 30°C.

外觀

Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

準備報告

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.