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Key Documents

SRP0218

Sigma-Aldrich

TC-PTP Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

同義詞:

PTPN2, T-cell protein-tyrosine phosphatase, TC-PTP

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

生物源

human

重組細胞

expressed in E. coli

化驗

≥90% (SDS-PAGE)

形狀

aqueous solution

分子量

62.3 kDa

包裝

pkg of 20 μg

儲存條件

avoid repeated freeze/thaw cycles

濃度

2.9 mg/mL

NCBI登錄號

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−70°C

基因資訊

human ... PTPN2(5771)

一般說明

Protein tyrosine phosphatase, Catalytic domain, (amino acids 2-315), (Gene bank accession number BC016727) with N-terminal GST tag, MW = 62.3 kDa, expressed in an E. coli expression system.

應用

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

單位定義

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

外觀

Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

準備報告

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Jiao Luo et al.
British journal of pharmacology, 177(19), 4464-4480 (2020-07-15)
Protein tyrosine phosphatase (PTP) 1B (PTP1B) plays a critical role in the regulation of obesity, Type 2 diabetes mellitus and other metabolic diseases. However, drug candidates exhibiting PTP1B selectivity and oral bioavailability are currently lacking. Here, the enzyme inhibitory characteristics

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