SRP0169

JMJD2B Active human

recombinant, expressed in baculovirus infected insect cells, ≥40% (SDS-PAGE)

• 同義詞: JHDM3B, JmjC domain-containing histone demethylation protein 3B, Jumonji domain-containing protein 2B, KIAA0876, Lysine-specific demethylase 4B (KDM4B)
• 分類程式碼代碼: 12352202
• NACRES: NA.32

屬性

• 生物源: human
• 重組細胞: expressed in baculovirus infected insect cells
• 化驗: ≥40% (SDS-PAGE)
• 形狀: aqueous solution
• 分子量: 57 kDa
• 包裝: pkg of 100 μg
• 濃度: >0.02 mg/mL
• NCBI登錄號: NM_015015
• UniProt登錄號: O94953
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... KDM4B(23030)

描述

一般說明

Human JMJD2B, also known as JHDM3B and KDM4B (GenBank Accession No.NM_015015) (amino acids 1-500) with C-terminal FLAG tag, MW = 57 kDa, expressed in Sf9 cells using a Baculovirus expression system.

應用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

單位定義

One unit is defined as the amount of enzyme required to demethylate 1 pmol of substrate/min at 37°C.

外觀

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

準備報告

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.