SRP0132

EZH2 / EED / SUZ12 human

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

• 同義詞: ENX-1, EZH2, Enhancer of zeste homolog 2, KMT6A, Lysine N-methyltransferase 6A
• 分類程式碼代碼: 12352200
• NACRES: NA.32

屬性

• 生物源: human
• 重組細胞: expressed in baculovirus infected insect cells
• 化驗: ≥90% (SDS-PAGE)
• 形狀: aqueous solution
• 分子量: 51 kDa (EED); 86 kDa (EZH2); 87 kDa (SUZ12)
• 包裝: pkg of 50 μg
• 濃度: >0.02 mg/mL
• NCBI登錄號: NM_003797 (EED); NM_004456 (EZH2); NM_015355 (SUZ12)
• UniProt登錄號: Q15910
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... EZH2(2146)

描述

一般說明

Complex of: Human EZH2 (GenBank Accession No. NM_004456), (amino acids 2-end) with N-terminal His tag, MW = 86 kDa. Human EED (GenBank Accession No. NM_003797) (amino acid 2-end) with N-terminal Flag tag, MW = 51 kDa. Human SUZ12, NM_015355, amino acids 2-end, with N-terminal His tag.

應用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

單位定義

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

外觀

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 100 μg/ml flag peptide and 10% glycerol.

準備報告

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.