SRE0117

Salt Active Nuclease (SAN)

High Purity, recombinant, expressed in Pichia pastoris, Triton-free

• 同義詞: endonuclease, salt active endonuclease, salt tolerant nuclease
• 分類程式碼代碼: 12352204
• NACRES: NA.54

屬性

• 重組細胞: expressed in Pichia pastoris
• 品質等級: 200
• 成份: Triton-free
• 包裝: pkg of 25 kU
• 運輸包裝: dry ice
• 儲存溫度: −20°C

描述

一般說明

This Salt Active Nuclease (SAN) is the ultimate solution for efficient removal of nucleic acids in manufacturing and bioprocessing workflows. This nonspecific, recombinant endonuclease has optimum activity at high salt concentrations, which can improve efficiency and yield in various workflows.

應用

• Useful for purification of tagged recombinant proteins
• Manufacturing of nucleic acid-free molecular enzymes and reagents
• Research of vaccines, viruses and antibodies

特點和優勢

• Active at low temperatures
• Binds strongly to cation exchange media because of high isoelectric point, even at elevated pH and salt concentrations where few other proteins show binding
• Typically does not bind to anion exchange or IMAC media
• Detergent free No protease detected

單位定義

One unit is defined as the amount of enzyme that causes a ΔA260 = 1.0 in 30 minutes at 37 °C in 25 mM Tris-HCI pH 8.5 (@25 °C), 5 mM MgCl₂, 500 mM NaCl, and 50 µg/mL calf thymus DNA.

重構

In solution

其他說明

• Temperature: 5 – 40°C, optimal: ~35°C
• Salt concentration (NaCl / KCl): 150 - 900 mM, optimal: 400 - 650 mM
• Mg²⁺: >1 mM is required for activity, optimal 5 - 50 mM
• pH: 7.3 – 10.0, optimal 8.2 - 9.2

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable