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重要文件

SHPMLIBR

Sigma-Aldrich

MISSION® LentiPlex® 完整小鼠混合shRNA库

For Rapid, Convenient Genome-wide shRNA Screens

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About This Item

分類程式碼代碼:
41105904
NACRES:
NA.51

產品線

MISSION®

濃度

≥5x108 VP/ml (via p24 assay)

技術

capture ELISA: 5 x 108 TU/mL using p24

運輸包裝

dry ice

儲存溫度

−70°C

相關類別

一般說明

MISSION LentiPlex 混合文库结合了MISSION TRC shRNA系列的强大能力与Sigma的慢病毒制造专业知识，可在您的工作台上实现全基因组RNAi筛选。LentiPlex混合系统可对非分裂和原代细胞系提供增强递送和长期基因沉默效果，可带来无限的探索潜力。这种方便的形式允许任何研究人员使用快速全基因组RNAi筛选方法，仅需极少的试剂、时间或资金设备投入。

MISSION LentiPlex 完整小鼠shRNA混合文库是使用专有工艺生产的全基因组慢病毒库。该文库包括110,000多个shRNA构建体，来自靶向21,000个小鼠基因的TRC集合。在产品发布之前，每个文库都要测试shRNA表达，以确保可靠的文库覆盖范围。文库以即用型慢病毒形式提供，通过p24检测，滴度至少为5 x 108 TU/ml，并预先分为15个子库，每个子库约有5,500 - 8000个shRNA构建体。另外提供用于下游靶标识别的扩增和测序引物。

有关更多信息，包括样品数据和用途，请访问LentiPlex技术详细信息页面。

其他說明

MISSION LentiPlex 小鼠混合shRNA文库包括货号SHPM01、SHPM15、SHPM2和SHPMLIBR的产品。请参阅表1了解每个文库相应的shRNA克隆库覆盖范围、靶标基因和子库数。

另外提供用于下游比中识别的扩增和测序引物。

shRNA库反卷积
Sigma反卷积服务可让您轻松识别影响混合shRNA筛选的基因。

  • 新一代克隆测序可精确测定混合样品中的单个克隆次数。
  • 确保混合shRNA筛选实现全面、可重现的结果
  • 稳健统计和信息丰富的数据

联系当地Sigma销售代表，了解更多信息或提交MISSION RNAi咨询

推薦產品

在我们的MISSION shRNA门户网站中浏览其他MISSION shRNA基因家族集

法律資訊

慢病毒许可协议
LentiPlex is a registered trademark of Merck KGaA, Darmstadt, Germany
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

相關產品

產品號碼
描述
訂價

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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分析證明 (COA)

Lot/Batch Number

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