SHC014V

MISSION^®

Contains a gene encoding TurboGFP, under the control of the UbC promoter

• 同義詞: MISSION^®, MISSION^® Control Transduction Particles
• 分類程式碼代碼: 41106609
• NACRES: NA.51

屬性

• 品質等級: 100
• 產品線: MISSION^®
• 濃度: ≥1x10⁶ VP/ml (via p24 assay)
• 技術: capture ELISA: 10⁶ TU/mL using p24
• 運輸包裝: dry ice
• 儲存溫度: −70°C

描述

一般說明

Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 10⁶ TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.

当使用MISSION^® TRC shRNA克隆进行实验时，选择适当对照品是您的实验设计的关键要素，以便准确解释敲低结果。 MISSION对照转导颗粒是监测转导效率的关键阳性对照。
想要查看更多应用数据、实验方案和载体图谱，请访问 sigma.com/shrna。

應用

To see more application data, protocols, vector maps visit sigma.com/shrna.

When conducting experiments using MISSION shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. Silencing of the CMV promoter can be a problem in some cell types. For these cells, the ubiquitin promoter (UbC) can be a viable alternative. The MISSION UbC-TurboGFP Control Transduction Particles are a positive control to monitor transduction efficiency for applications in which an alternate promoter is necessary. The MISSION UbC-TurboGFP Control Transduction Particles are produced from the sequence-verified 9092 base pair lentivirus plasmid, pLKO.1-puro-UbC-TurboGFP (SHC014).

This construct aids in interpretation of experimental design and results by providing a green fluorescent protein marker for monitoring success in transduction of your cells of interest.

法律資訊

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

TurboGFP is a trademark of Evrogen Co.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable