SHC004H

MISSION^®

High Titer, shRNA sequence targeting tGFP

• 同義詞: MISSION^®, MISSION^® Control Transduction Particles
• 分類程式碼代碼: 41106609
• NACRES: NA.51

屬性

• 產品線: MISSION^®
• 濃度: ≥1x10⁹ VP/ml (via p24 assay)
• 技術: capture ELISA: 10⁹ TU/mL using p24
• 運輸包裝: dry ice
• 儲存溫度: −70°C

描述

一般說明

当使用MISSION^® TRC shRNA克隆进行实验时，选择适当对照品是您的实验设计的关键要素，以便准确解释敲低结果。 MISSION对照转导颗粒是监测转导效率的关键阳性对照。
想要查看更多应用数据、实验方案和载体图谱，请访问 sigma.com/shrna。

The MISSION^® TurboGFP^™ shRNA Control Transduction Particles contain an shRNA sequence targeting TurboGFP. The TurboGFP shRNA Control Particles are useful as a positive knockdown control in experiments using the MISSION TurboGFP positive control vector or in cell lines expressing TurboGFP. TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.

The TurboGFP shRNA control transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-TurboGFP shRNA (SHC004). Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 10⁹ TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.

This high titer offering is recommended for customers: who need to utilize smaller volumes of virus, who have very difficult to transduce cell lines, or who need to transduce large numbers of cells.

應用

To see more application data, protocols, vector maps visit sigma.com/shrna.

成分

High titer controls are provided as 10 x 20 μl aliquots.

法律資訊

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

TurboGFP is a trademark of Evrogen Co.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable