SAE0217

Enhanced Microbial Transglutaminase

Glycosylation tolerant, for site-specific antibody bioconjugation

• 同義詞: Protein-Glutamine-γ-Glutamyltransferase, Protein-glutamine:amine γ-glutamyltransferase, eMTG
• 分類程式碼代碼: 12352204

屬性

• 重組細胞: expressed in E. coli
• 品質等級: 200
• 比活性: ≥30 units/mg protein
• 運輸包裝: dry ice
• 儲存溫度: -10 to -25°C

描述

一般說明

eMTG (Enhanced Microbial Transglutaminase) is an enzyme that catalyzes the formation of new isopeptide bonds between the primary amine of a drug linker and glutamine residues at position Q295 of IgG-type antibodies to produce site-specifically conjugated antibody-drug conjugates (ADCs).

應用

Site-specific conjugation is increasingly used in drug research, development, and manufacturing to produce ADCs and bioconjugates with defined drug-to-antibody ratios (DAR) since the resulting homogeneity improves safety and efficacy. Transglutaminase (TG)-mediated bioconjugation is a promising means of conjugating drug linkers specifically to the Q295 position of antibodies. However, its use traditionally requires antibody or glycan engineering since the adjacent glycosylation at N297 interferes with the TG reaction. If you are interested in trying eMTG to make therapeutic ADCs but do not currently have the conjugation setup/expertise, please consider ADC Express™ Service for pre-clinical lead candidate selection.

特點和優勢

• Increased efficiency - with one-step bioconjugation for ADCs

• Glycosylation-tolerant - no need to remove glycosylation allowing for a preserved glycan on the antibody conjugate product

• Built for purpose - eMTG has been designed for producing ADCs and bioconjugates

• For higher-grade quality suitable for cGMP manufacturing of ADCs, please reach out using this form https://www.sigmaaldrich.com/US/en/services/contract-manufacturing/adc-bioconjugation/adc-bioconjugation-request-information with eMTG selected.

單位定義

One unit will catalyze the formation of 1.0 micromole of hydroxamate per minute from N Z GLN-GLY and hydroxylamine at pH 6.0 at 37°C.

安全資訊

• 象形圖: GHS08
• 訊號詞: Danger
• 危險聲明: H334
• 防範說明: P261 - P284 - P501
• 危險分類: Resp. Sens. 1
• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1