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Merck
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重要文件

SAE0213

Sigma-Aldrich

Arylsulfatase, Enhanced, Recombinant

From P. aeruginosa for Steroid Sulfate Hydrolysis

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About This Item

酶委員會編號:
EC 3.1.6.1
分類程式碼代碼:
12352204
NACRES:
NA.77

品質等級

形狀

liquid

比活性

≥100 units/mg protein

分子量

60 kDa

運輸包裝

dry ice

儲存溫度

-10 to -25°C

一般說明

Enhanced arylsulfatase is a recombinantly expressed form of the arylsulfatase found in Pseudomonas arigenosa, with introduced mutations for more efficient catalysis of steroidal sulfate esters. It is useful for sample preparation of urine metabolites (sulfated drug and endogenous conjugates) before analysis by HPLC and/or Mass Spec. It will hydrolyze sulfoconjugates to improve retention time and consolidate parent analyte peaks on HPLC.

應用

Useful for endogenous and xenobiotic and drug metabolite analysis.

生化/生理作用

(Aryl)sulfatases are used by the body for the catabolism of sulfated compounds, which play a crucial role in a number of physiological processes. Some examples include sulfated glucosaminoglycans like heparin, hyaluronan and chondroitin. This particular arylsulfatase is derived from microbial source and is used as a tool for processing sulfated metabolites, rather than for its physiological purpose.

外觀

Supplied as buffered solution containing 20 mM Hepes pH7.8, 150 mM NaCl, 10% v/v Glycerol and 0.5 mM CaCl2.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2


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