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Merck
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重要文件

SAE0103

Sigma-Aldrich

A2A (Adenosine Receptor A2A)

recombinant, expressed in Sf9 cells

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.26

重組細胞

expressed in Sf9 cells

描述

N-Terminal contains a strep tag II and 10X Histidine tag followed by a TEV protease cleavage site.

化驗

≥90% (SDS-PAGE)

形狀

aqueous solution

分子量

47.7 kDa

濃度

1.72 mg/mL

NCBI登錄號

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−70°C

生化/生理作用

A2A is a class A GPCR involved in regulating myocardial blood flow and hypertension.

序列

MWSHPQFEKHHHHHHHHENLYFQGPIMGSSVYITVELAIAVLAILGNVLVCWAVWLNSNLQNVTNYFVVSLAAADIAVGVLAIPFAITISTGFCAACHGCLFIACFVLVLTQSSIFSLLAIAIDRYIAIRIPLRYNGLVTGTRAKGIIAICWVLSFAIGLTPMLGWNNCGQPKEGKNHSQGCGEGQVACLFEDVVPMNYMVYFNFFACVLVPLLLMLGVYLRIFLAARRQLKQMESQPLPGERARSTLQKEVHAAKSLAIIVGLFALCWLPLHIINCFTFFCPDCSHAPLWLMYLAIVLSHTNSVVNPFIYAYRIREFRQTFRKIIRSHVLRQQEPFKAAGTSARVLAAHGSDGEQVSLRLNGHPPGVWANGSAPHPERRPNGYALGLVSGGSAQESQGNTGLPDVELLSHELKGVCPEPPGLDDPLAQDGAGVS

準備報告

50mM Hepes pH 7.4, 200mM NaCl, 0.05%/0.006% DDM/CHS

儲存和穩定性

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot into single use aliquots. Store remaining undiluted protein in aliquots at -70°C.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析證明 (COA)

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Frontiers in pharmacology, 8, 898-898 (2018-01-10)
Adenosine receptors (ARs) comprise the P1 class of purinergic receptors and belong to the largest family of integral membrane proteins in the human genome, the G protein-coupled receptors (GPCRs). ARs are classified into four subtypes, A1, A2A, A2B, and A3

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