SAB5900202

Alpaca Anti-PD-L1, recombinant single domain Antibody

Recombinant Alpaca(VHH) Nano antibody

• 同義詞: B7-H, B7H1, PD-L1, PDCD1LG1, PDL1
• 分類程式碼代碼: 12352203
• NACRES: NA.43

屬性

• 生物源: alpaca
• 品質等級: 100
• 重組細胞: expressed in E. coli
• 共軛: unconjugated
• 抗體表格: affinity purified VHH antibody
• 無性繁殖: PL03, monoclonal
• 化驗: >95% (SDS-PAGE)
• 形狀: lyophilized solid
• 分子量: 14.4 kDa; ~ 15-18 kDa (SDS PAGE under reducing condition)
• 純化經由: immobilized metal affinity chromatography (IMAC)
• 物種活性: human, (Cynomolgus macaques)
• 濃度: 1 mg/mL
• 技術: ELISA: 1:10000-1:100000; flow cytometry: 1:50-1:100; immunofluorescence: 1:200-1:800; immunohistochemistry: 1:200-1:800; western blot: 1:1000-1:3000
• 顏色: colorless
• pH值: ( in PBS, pH 7.4)
• 同型: VHH
• Protein ID登錄號: Q9NZQ7.1
• UniProt登錄號: Q9NZQ7
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... CD274(29126)

描述

一般說明

PD-L1, also known as CD274 or B7H1, stands for programmed cell death ligand 1. It is a type I transmembrane protein that is thought to repress immune responses by binding to its receptor (PD1), thus inhibiting T-cell activation, proliferation, and cytokine production. It contains V-like and C-like immunoglobulin domains. PD-L1 expression is regulated by various cytokines, such as TNF-α or LPS (ISSN: 1848-7718). Increased expression of this protein in certain types of cancers, e.g., renal cell carcinoma or colon cancer, correlates with poor prognosis.Alpaca derived anti-PD-L1 VHH single domain antibody (molecular weight: 14.4kDa), expressed in E. coli under conditions free from animal derived components. It has a 6His tag at its C-terminal.
Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. The small size and monovalency makes it a superior tool in microsopy. These single-domain antibodies offer superior chemical (wide variety of pH, urea, etc) and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility.

特異性

Human Programmed Death-Ligand 1 (PD-L1)

免疫原

Recombinant human PD-L1 (Phe19-Tyr134) protein

應用

ELISA, WB, FC, IHC, IF

包裝

50 µg per vial / 100 µg per vial

外觀

Affinity purified VHH single domain antibody lyophilized from PBS, pH7.5

重構

Reconstitute the lyophilized antibody with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.

儲存和穩定性

Stable for 1 year at 4°C from date of receipt.

其他說明

Storage at 4°C; Do not freeze.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable