SAB4200597

Monoclonal Anti-FMR1 antibody produced in mouse

clone FMR2.5, hybridoma cell culture supernatant

• 同義詞: FMRP, FRAXA, POF, POF1, fragile X mental retardation 1
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: mouse
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: FMR2.5, monoclonal
• 形狀: buffered aqueous solution
• 分子量: ~71 kDa
• 物種活性: rat, human, mouse, hamster
• 濃度: ~1 mg/mL
• 技術: flow cytometry: 2.0-5.0 μg/test using HeLa cells; immunoblotting: 1.0-2.0 μg/mL using HepG2 total cell extracts.; immunocytochemistry: suitable; immunofluorescence: 5-10 μg/mL using HepG2 cells.
• 同型: IgG1
• UniProt登錄號: Q06787
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... FMR1(2332) ; mouse ... Fmr1(14265) ; rat ... Fmr1(24948)

描述

一般說明

Monoclonal Anti-FMR1 (mouse IgG1 isotype) is derived from the hybridoma FMR2.5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Fragile X mental retardation 1 (FMR1) is encoded by the gene mapped to human chromosome Xq27.3q28. FMR1 localizes to both the nucleus and the cytoplasm.

特異性

Monoclonal Anti- FMR1 recognizes human, hamster, rat and mouse FMR1.

免疫原

synthetic peptide corresponding to a sequence at the C-terminal region of human FMR1

應用

Monoclonal Anti-FMR1 antibody produced in mouse may be used in:

• immunoblotting
• immunocytochemistry
• immunofluorescence
• flow cytometry

生化／生理作用

Fragile X mental retardation 1 (FMR1) protein functions as an RNA-binding protein that associates with polyribosomes and is a likely component of a messenger ribonuclear protein (mRNP) particle. Since, FMR1 contains both a nuclear localization signal and a nuclear export signal it is also implicated in nucleocytoplasmic transport. Mutation in the gene leads to the development of fragile X mental retardation (FXMR) syndrome.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

儲存和穩定性

For extended storage, freeze at 20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: nwg
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable