MAB2252

Anti-Integrin β1 Antibody, clone N29

clone N29, Chemicon^®, from mouse

• 同義詞: CD29, MAB2252Z
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: N29, monoclonal
• 物種活性: human
• 製造商／商標名: Chemicon^®
• 技術: cell culture | mammalian: suitable; flow cytometry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgGκ
• NCBI登錄號: NM_002292.3
• UniProt登錄號: P55268
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ITGB1(3688)

描述

特異性

Reacts with the human integrin beta1 subunit. Specificity verified by preclearing of beta1in immunoprecipitation, flow cytometry on transfectant cells displaying human beta1 integrin, and reactivity with purified beta1. Recognizes an epitope cluster distinct from MAB2250 and MAB2251(Wilkins et al., 1996).

免疫原

Jurkat T-leukemic cell line

應用

Immunohistochemistry (Paraffin) Analysis: A 1:50-2,000 dilution from a representative lot detected Integrin β1 in the membrane of tubule epithelial cells and cells of the glomeruli in human kidney tissue, membrane of alveolar cells, endothelial cells, and bronchiole epithelial cells of human lung tissue.

Immunoprecipitation: A representative lot of this antibody clone was used in immunoprecipitation.

Immunohistochemistry: A representative lot of this antibody clone was used in immunohistochemistry on frozen sections.

Flow Cytometry: A representative lot of this antibody clone was used in flow cytometry.

Functional Activity Assay: A representative lot of this antibody clone was used to stimulate adhesion of cells to extracellular matrix proteins (Wilkins, J.A. et al., 1996).

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Integrins

This Anti-Integrin β1 Antibody, clone N29 is validated for use in FC, IH, IP, FUNC, CULT, WB for the detection of Integrin β1.

品質

Western Blot Analysis: 0.5 µg/mL of the antibody detected Integrin β1 in 10 µg of U251 cell lysate.

標靶描述

88 kDa

外觀

Protein A purified

Format: Purified

Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.

儲存和穩定性

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

分析報告

Control
Tonsil, human skin, human kidney tissue

U251 cell lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable